Anti-SPARCL1 Antibody | Boster Immunoleader PA2088 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-SPARCL1 Antibody

catalog :

PA2088

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

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product information

Availability :

info changed 20150327

Product Name :

Anti-SPARCL1 Antibody

Catalog Number :

PA2088

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for SPARC-like protein 1(SPARCL1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, mouse, rat

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Mouse, Rat

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human SPARCL1(649-664aa HCFGIKEEDIDENLLF), identical to the related mouse and rat sequences.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

SPARCL1(SPARC-Like Protein 1), also known as HEVIN, is a protein that in humans is encoded by the SPARCL1 gene. The cells in high endothelial venules(HEVs) in lymphoid tissues have a plump, almost cuboidal, appearance and support high levels of lymphocyte extravasation from blood, possibly due to the presence of desmosome-like junctions rather than tight junctions in the HEVs. In chronic inflammation, the activated endothelium of nonlymphoid tissues acquires an HEV-like morphology and function. Hevin is highly expressed in HEV and is though to contribute to the induction or maintenance of features of the HEV endothelium that facilitate lymphocyte migration(Girard and Springer, 1995).

Reference :

1. Girard, J.-P., Springer, T. A. Cloning from purified high endothelial venule cells of hevin, a close relative of the antiadhesive extracellular matrix protein SPARC. Immunity 2: 113-123, 1995.

Gene Name :

SPARCL1

Protein Name :

SPARC-like protein 1

Gene Full Name :

SPARC-like 1(hevin)

Synonyms :

Ecm2 antibody Extracellular matrix protein 2 antibody Hevin antibody High endothelial venule protein antibody MAST 9 antibody mast9 antibody Matrix glycoprotein Sc1 antibody PIG33 antibody proliferation inducing protein 33 antibody RAGS1 antigen antibody SC1 antibody SPARC like 1 antibody SPARC-like protein 1 antibody Sparcl1 antibody SPRL1_HUMAN antibody

Uniprot ID :

Q14515

more info or order :

Boster Immunoleader product webpage