Boster Immunoleader

antibody

Anti-Prolactin Receptor Antibody

PA2087

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse, rat

western blot, immunohistochemistry - paraffin section

info changed 20150327

Anti-Prolactin Receptor Antibody

PA2087

Polyclonal

Rabbit IgG polyclonal antibody for Prolactin receptor(PRLR) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, rat Predicted to work with: mouse

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Human; Predicted Species: Mouse, Rat

Concentration: 0.5-1μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the C-terminus of mouse Prolactin Receptor(591-605aa QLGRLDYLDPTCFMH), identical to the related rat sequence, and different from the related human sequence by three amino acids.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

PRLR(Prolactin Receptor), is a cytokine receptor. By somatic cell hybrid analysis and by in situ hybridization, Arden et al.(1989, 1990) demonstrated that the prolactin receptor gene resides in the same chromosomal region as the growth hormone receptor gene, which has been mapped to 5p13-p12. Cunningham et al.(1990) demonstrated that zinc greatly increases the affinity of GH for the extracellular binding domain of PRLR, although it is not required for binding of GH to the growth hormone receptor or for binding of prolactin to the prolactin receptor. By mutational analysis, they showed that a cluster of 3 residues(histidine-18, histidine-21, and glutamic acid-174) in GH and histidine-188 in PRLR(conserved in all PRL receptors but not GH receptors) are likely zinc-ion ligands.

1. Arden, K. C., Boutin, J.-M., Djiane, J., Kelly, P. A., Cavenee, W. K. The receptors for prolactin and growth hormone are localized in the same region of human chromosome 5. Cytogenet. Cell Genet. 53: 161-165, 1990. 2. Arden, K. C., Cavenee, W. K., Boutin, J.-M., Kelly, P. A. The genes encoding the receptors for prolactin and growth hormone map to human chromosome 5. (Abstract) Am. J. Hum. Genet. 45 (suppl.): A129 only, 1989. 3. Cunningham, B. C., Bass, S., Fuh, G., Wells, J. A. Zinc mediation of the binding of human growth hormone to the human prolactin receptor. Science 250: 1709-1712, 1990.

PRLR

Prolactin receptor

prolactin receptor

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Q08501