Boster Immunoleader

antibody

Anti-Proprotein Convertase 2 Antibody

PA2085

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse, rat

western blot, immunohistochemistry - paraffin section

Anti-Proprotein Convertase 2 Antibody

PA2085

Polyclonal

Rabbit IgG polyclonal antibody for Neuroendocrine convertase 2(PCSK2) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, rat Predicted to work with: mouse

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Human; Predicted Species: Mouse, Rat

Concentration: 0.5-1μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

WB: The detection limit for PCSK2 is approximately 10ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the N-terminus of human Proprotein Convertase 2(110-125aa GYRDINEIDINMNDPL), identical to the related mouse and rat sequences.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

PCSK2(Proprotein Convertase, Subtilisin/Kexin-Type, 2), also known as PC2 or NEC2, is an enzyme responsible for the first step in the maturation of many neuroendocrine peptides from their precursors, such as the conversion of proinsulin to insulin intermediates. Seidah et al.(1991) mapped the NEC2 gene to human chromosome 20p11.2-p11.1. Ohagi et al.(1992) observed expression of the chloramphenicol acetyltransferase reporter fusions containing the putative promoter region in insulinoma cells but not in hepatoma cells, consistent with the known tissue-specific pattern of expression of the PC2 gene. Analysis of the level of CAT activity with several deletion mutants identified the region from -1100 to -539 from the translation start site as essential for PC2 promoter activity.

1. Maglott, D. R., Feldblyum, T. V., Durkin, A. S., Nierman, W. C. Radiation hybrid mapping of SNAP, PCSK2, and THBD (human chromosome 20p). Mammalian Genome 7: 400-401, 1996. 2. Ohagi, S., LaMendola, J., LeBeau, M. M., Espinosa, R., III, Takeda, J., Smeekens, S. P., Chan, S. J., Steiner, D. F. Identification and analysis of the gene encoding human PC2, a prohormone convertase expressed in neuroendocrine tissues. Proc. Nat. Acad. Sci. 89: 4977-4981, 1992. 3. Seidah, N. G., Mattei, M. G., Gaspar, L., Benjannet, S., Mbikay, M., Chretien, M. Chromosomal assignments of the genes for neuroendocrine convertase PC1 (NEC1) to human 5q15-21, neuroendocrine convertase PC2 (NEC2) to human 20p11.1-11.2, and furin (mouse 7[D1-E2] region). Genomics 11: 103-107, 1991.

PCSK2

Neuroendocrine convertase 2

proprotein convertase subtilisin/kexin type 2

KEX2 like endoprotease 2 antibody KEX2-like endoprotease 2 antibody NEC 2 antibody NEC2 antibody NEC2_HUMAN antibody Neuroendocrine convertase 2 antibody PC2 antibody PCSK2 antibody Prohormone convertase 2 antibody Proprotein convertase 2 antibody Proprotein convertase subtilisin/kexin type 2 antibody SPC2 antibody

P16519