Anti-MAdCAM1 Antibody | Boster Immunoleader PA2083 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-MAdCAM1 Antibody

catalog :

PA2083

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

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Boster Immunoleader product webpage

product information

Product Name :

Anti-MAdCAM1 Antibody

Catalog Number :

PA2083

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Mucosal addressin cell adhesion molecule 1(MADCAM1) detection. Tested with WB, IHC-P, IHC-F in Human.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human

Applications :

WB,IHC-P,IHC-F

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human

Immunohistochemistry(Frozen Section) :

Concentration: 0.5-1μg/ml; Tested Species: Human

Notes :

WB: The detection limit for MADCAM1 is approximately 0.5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence in the middle region of human MAdCAM1(173-190aa EEPQGDEDVLFRVTERWR).

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P) and IHC(F).

Background :

MADCAM1(Mucosal Vascular Addressin Cell Adhesion Molecule 1), also known as MACAM1, is a protein that in humans is encoded by the MADCAM1 gene. By PCR-based analysis of somatic cell hybrids, Leung et al.(1997) mapped the MACAM1 gene to chromosome 19. The protein encoded by this gene is an endothelil cell adhesion molecule that interacts preferentially with the leukocyte beta7 integrin LPAM-1(alpha4 / beta7), L-selectin, and VLA-4(alpha4/beta1) on myeloid cells to direct leukocytes into mucosal and inflamed tissues. It is a member of the immunoglobulin superfamily and is similar to ICAM-1 and VCAM-1.

Reference :

1. Briskin, M. J., McEvoy, L. M., Butcher, E. C. MAdCAM-1 has homology to immunoglobulin and mucin-like adhesion receptors and to IgA1. Nature 363: 461-464, 1993. 2. Entrez Gene: mucosal vascular addressin cell adhesion molecule 1. 3. Leung, E., Berg, R. W., Langley, R., Greene, J., Raymond, L. A., Augustus, M., Ni, J., Carter, K. C., Spurr, N., Choo, K. H. A., Krissansen, G. W. Genomic organization, chromosomal mapping, and analysis of the 5-prime promoter region of the human MAdCAM-1 gene. Immunogenetics 46: 111-119, 1997.

Gene Name :

MADCAM1

Protein Name :

Mucosal addressin cell adhesion molecule 1

Gene Full Name :

mucosal vascular addressin cell adhesion molecule 1

Synonyms :

Addressin mucosal antibody hMAdCAM 1 antibody hMAdCAM-1 antibody MACAM1 antibody MADCA_HUMAN antibody MAdCAM 1 antibody MAdCAM-1 antibody Madcam1 antibody Mucosal addressin cell adhesion molecule 1 antibody Mucosal vascular addressin cell adhesion molecule 1 antibody

Uniprot ID :

Q13477

more info or order :

Boster Immunoleader product webpage