Anti-ADAMTS5 Antibody | Boster Immunoleader PA2072 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-ADAMTS5 Antibody

catalog :

PA2072

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-ADAMTS5 Antibody

Catalog Number :

PA2072

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for A disintegrin and metalloproteinase with thrombospondin motifs 5(ADAMTS5) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, rat Predicted to work with: mouse

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Rat; Predicted Species: Human, Mouse

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human ADAMTS5(772-787aa TAYLALKKKNGEYLIN), different from the related mouse and rat sequence by one amino acid.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

ADAMTS5(A Disintegrin-Like and Metalloproteinase with Thrombospondin Type 1 Motif, 5), is an enzyme that in humans is encoded by the ADAMTS5 gene. ADAMTS5 is a member of the large ADAMTS family of zinc-dependent proteases. The enzyme encoded by this gene contains two C-terminal TS motifs and functions as aggrecanase to cleave aggrecan, a major proteoglycan of cartilage. By somatic cell hybrid analysis, Hurskainen et al.(1999) mapped the human ADAMTS5 gene to chromosome 21. Using mouse models, Echtermeyer et al.(2009) showed that Sdc4 controls a pathway that activates Adamts5 at the chondrocyte cell surface through Erk1/Erk2 activation of Mmp3.

Reference :

1. Echtermeyer, F., Bertrand, J., Dreier, R., Meinecke, I., Neugebauer, K., Fuerst, M., Lee, Y. J., Song, Y. W., Herzog, C., Theilmeier, G., Pap, T. Syndecan-4 regulates ADAMTS-5 activation and cartilage breakdown in osteoarthritis. Nature Med. 15: 1072-1076, 2009. 2. Glasson, S. S., Askew, R., Sheppard, B., Carito, B., Blanchet, T., Ma, H.-L., Flannery, C. R., Peluso, D., Kanki, K., Yang, Z., Majumdar, M. K., Morris, E. A. Deletion of active ADAMTS5 prevents cartilage degradation in a murine model of osteoarthritis. Nature 434: 644-648, 2005. Note: Erratum: Nature 446: 102 only, 2007. 3. Hurskainen, T. L., Hirohata, S., Seldin, M. F., Apte, S. S. ADAM-TS5, ADAM-TS6, and ADAM-TS7, novel members of a new family of zinc metalloproteases: general features and genomic distribution of the ADAM-TS family. J. Biol. Chem. 274: 25555-25563, 1999.

Gene Name :

ADAMTS5

Protein Name :

A disintegrin and metalloproteinase with thrombospondin motifs 5

Gene Full Name :

ADAM metallopeptidase with thrombospondin type 1 motif, 5

Synonyms :

A disintegrin and metalloproteinase with thrombospondin motifs 11 antibody A disintegrin and metalloproteinase with thrombospondin motifs 5 antibody A disintegrin like and metalloprotease(reprolysin type) with thrombospondin type 1 motif 5 antibody A disintegrin like and metalloprotease(reprolysin type) with thrombospondin type 1 motif, 5(aggrecanase 2) antibody A Disintigrin And Metalloproteinase with ThromboSpondin motif-5 antibody ADAM metallopeptidase with thrombospondin type 1 motif 5 antibody ADAM TS 11 antibody ADAM TS 5 antibody ADAM TS5 antibody ADAM-TS 11 antibody ADAM-TS 5 antibody ADAM-TS5 antibody ADAMTS 11 antibody ADAMTS 5 antibody ADAMTS-11 antibody ADAMTS-5 antibody ADAMTS11 antibody Adamts5 antibody ADMP 2 antibody ADMP-2 antibody ADMP2 antibody Aggrecanase 2 antibody Aggrecanase-2 antibody ATS5_HUMAN antibody FLJ36738 antibody Implantin antibody ThromboSpondin motif-5 antibody

Uniprot ID :

Q9UNA0

more info or order :

Boster Immunoleader product webpage