Anti-PROM1 Antibody | Boster Immunoleader PA2063 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-PROM1 Antibody

catalog :

PA2063

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

mouse, rat

application :

western blot

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-PROM1 Antibody

Catalog Number :

PA2063

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Prominin-1(PROM1) detection. Tested with WB in Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: mouse, rat

Applications :

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Mouse, Rat

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of mouse PROM1(850-864aa KDHLYGVHNPVMTSP), identical to the related rat sequence.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Background :

PROM1(Prominin 1), also called CD133, is a glycoprotein. Maw et al.(2000) noted that D4S1601 and D4S3048, which are located on human chromosome 4p15.3-p15.2, map within intronic sequences of the PROML1 gene. Torrente et al.(2004) reported that a subpopulation of circulating cells expressing AC133, a well-characterized marker of hematopoietic stem cells, also expresses early myogenic markers. Singh et al.(2003) prospectively isolated a CD133-positive cell subpopulation from human brain tumors that exhibited stem cell properties in vitro. However, the true measures of cancer stem cells are their capacity for self renewal and exact recapitulation of the original tumor.

Reference :

1. Maw, M. A., Corbeil, D., Koch, J., Hellwig, A., Wilson-Wheeler, J. C., Bridges, R. J., Kumaramanickavel, G., John, S., Nancarrow, D., Roper, K., Weigmann, A., Huttner, W. B., Denton, M. J. A frameshift mutation in prominin (mouse)-like 1 causes human retinal degeneration. Hum. Molec. Genet. 9: 27-34, 2000. 2. Singh, S. K., Clarke, I. D., Terasaki, M., Bonn, V. E., Hawkins, C., Squire, J., Dirks, P. B. Identification of a cancer stem cell in human brain tumors. Cancer Res. 63: 5821-5828, 2003. 3. Torrente, Y., Belicchi, M., Sampaolesi, M., Pisati, F., Meregalli, M., D'Antona, G., Tonlorenzi, R., Porretti, L., Gavina, M., Mamchaoui, K., Pellegrino, M. A., Furling, D., Mouly, V., Butler-Browne, G. S., Bottinelli, R., Cossu, G., Bresolin, N. Human circulating AC133(+) stem cells restore dystrophin expression and ameliorate function in dystrophic skeletal muscle. J. Clin. Invest. 114: 182-195, 2004.

Gene Name :

PROM1

Protein Name :

Prominin-1

Gene Full Name :

prominin 1

Synonyms :

AC133 antibody Antigen AC133 antibody CD133 antibody CORD12 antibody hematopoietic stem cell antigen antibody hProminin antibody MCDR2 antibody MSTP061 antibody PROM1 antibody PROM 1 antibody PROM-1 antibody Prominin-1 antibody Prominin 1 antibody Prominin1 antibody Prominin-like protein 1 antibody PROML1 antibody RP41 antibody STGD4 antibody

Uniprot ID :

O54990

more info or order :

Boster Immunoleader product webpage