Anti-Factor VII Antibody | Boster Immunoleader PA2060 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Factor VII Antibody

catalog :

PA2060

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human

application :

western blot

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Factor VII Antibody

Catalog Number :

PA2060

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Coagulation factor VII(F7) detection. Tested with WB in Human.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human

Applications :

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Notes :

WB: The detection limit for F7 is approximately 2.5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human Factor VII(441-453aa SQYIEWLQKLMRS).

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Background :

F7(Coagulation Factor VII), also known as proconvertin, is one of the proteins that causes blood to clot in the coagulation cascade. It is an enzyme of the serine protease class. The F7 gene maps to chromosome 13q34(Millar et al., 2000). Synthesis of factors VII and X, as well as factors II and IX, takes place in the liver and requires vitamin K. Structural homologies of these factors, which are precursors of serine proteases, have been shown(Zur and Nemerson, 1981). Di Bitondo et al.(2002) used reporter gene analysis to show that inclusion of promoter regions of F7 reduced transcription activity in the presence of estrogenic factors. The effect was independent of promoter polymorphic haplotype.

Reference :

1. Di Bitondo, R., Hall, A. J., Peake, I. R., Iacoviello, L., Winship, P. R. Oestrogenic repression of human coagulation factor VII expression mediated through an oestrogen response element sequence motif in the promoter region. Hum. Molec. Genet. 11: 723-731, 2002. 2. Millar, D. S., Kemball-Cook, G., McVey, J. H., Tuddenham, E. G. D., Mumford, A. D., Attock, G. B., Reverter, J. C., Lanir, N., Parapia, L. A., Reynaud, J., Meili, E., von Felton, A., Martinowitz, U., Prangnell, D. R., Krawczak, M., Cooper, D. N. Molecular analysis of the genotype-phenotype relationship in factor VII deficiency. Hum. Genet. 107: 327-342, 2000. 3. Zur, M., Nemerson, Y. Tissue factor pathways of blood coagulation.In: Bloom, A. L.; Thomas, D. P. : Haemostasis and thrombosis. Edinburgh: Churchill Livingstone (pub.) 1981.

Gene Name :

Protein Name :

Coagulation factor VII

Gene Full Name :

coagulation factor VII(serum prothrombin conversion accelerator)

Synonyms :

coagulation factor VII(serum prothrombin conversion accelerator) antibody Coagulation factor VII antibody Eptacog alfa antibody F7 antibody FA7_HUMAN antibody Factor VII antibody Factor VII heavy chain antibody Factor VII light chain antibody FVII coagulation protein antibody OTTHUMP00000018733 antibody OTTHUMP00000018734 antibody Proconvertin antibody Serum prothrombin conversion accelerator antibody SPCA antibody

Uniprot ID :

P08709

more info or order :

Boster Immunoleader product webpage