Anti-CD244 Antibody | Boster Immunoleader PA2057 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-CD244 Antibody

catalog :

PA2057

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

more info or order :

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product information

Product Name :

Anti-CD244 Antibody

Catalog Number :

PA2057

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Natural killer cell receptor 2B4(CD244) detection. Tested with WB, IHC-P in Human.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human CD244(357-370aa RLSRKELENFDVYS).

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

CD244(Cluster of Differentiation 244) is a human protein encoded by the CD244 gene. It is also known as Natural Killer Cell Receptor 2B4. Tangye et al.(1999) mapped the 2B4 gene to 1q22. Suzuki et al.(2008) identified a functional single-nucleotide polymorphism(SNP) in the CD244 gene that contributes to rheumatoid arthritis susceptibility. Functional analysis by Boles et al.(1999) demonstrated that engagement of 2B4 with specific antibody activates NK cytolytic activity. Using recombinant human NK cell-activating ligand 2B4 fused to domains 3 and 4 of rodent Cd4 and flow cytometric analysis, Brown et al.(1998) demonstrated that CD48 binds to 2B4. Watzl et al.(2000) showed that antibody-mediated cross-linking of 2B4 leads to its rapid tyrosine phosphorylation, which is necessary for 2B4-mediated killer cell activity.

Reference :

1. Boles, K. S., Nakajima, H., Colonna, M., Chuang, S. S., Stepp, S. E., Bennett, M., Kumar, V., Mathew, P. A. Molecular characterization of a novel human natural killer cell receptor homologous to mouse 2B4. Tissue Antigens 54: 27-34, 1999. 2. Brown, M. H., Boles, K., van der Merwe, P. A., Kumar, V., Mathew, P. A., Barclay, A. N. 2B4, the natural killer and T cell immunoglobulin superfamily surface protein, is a ligand for CD48. J. Exp. Med. 188: 2083-2093, 1998. 3. Suzuki, A., Yamada, R., Kochi, Y., Sawada, T., Okada, Y., Matsuda, K., Kamatani, Y., Mori, M., Shimane, K., Hirabayashi, Y., Takahashi, A., Tsunoda, T., Miyatake, A., Kubo, M., Kamatani, N., Nakamura, Y., Yamamoto, K. Functional SNPs in CD244 increase the risk of rheumatoid arthritis in a Japanese population. Nature Genet. 40: 1224-1229, 2008. 4. Tangye, S. G., Lazetic, S., Woollatt, E., Sutherland, G. R., Lanier, L. L., Phillips, J. H. Cutting edge: human 2B4, an activating NK cell receptor, recruits the protein tyrosine phosphatase SHP-2 and the adaptor signaling protein SAP. J. Immun. 162: 6981-6985, 1999. 5. Watzl, C., Stebbins, C. C., Long, E. O. Cutting edge: NK cell inhibitory receptors prevent tyrosine phosphorylation of the activation receptor 2B4 (CD244). J. Immun. 165: 3545-3548, 2000.

Gene Name :

CD244

Protein Name :

Natural killer cell receptor 2B4

Gene Full Name :

CD244 molecule, natural killer cell receptor 2B4

Synonyms :

2B4 antibody C9.1 antibody CD244 antibody CD244 antigen antibody CD244 molecule natural killer cell receptor 2B4 antibody CD244 natural killer cell receptor 2B4 antibody CD244_HUMAN antibody F730046O15Rik antibody h2B4 antibody Ly90 antibody NAIL antibody Natural killer cell activation-inducing ligand antibody Natural killer cell receptor 2B4 antibody NK cell activation inducing ligand antibody NK cell activation inducing ligand NAIL antibody NK cell activation-inducing ligand antibody NK cell type I receptor protein 2B4 antibody NKR2B4 antibody Nmrk antibody Non-MHC restricted killing associated antibody OTTHUMP00000027884 antibody p38 antibody SLAM family, member 4 antibody SLAMF4 antibody

Uniprot ID :

Q9BZW8

more info or order :

Boster Immunoleader product webpage