Boster Immunoleader

antibody

Anti-Tenascin C Antibody

PA2055

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse

western blot, immunohistochemistry - paraffin section

info changed 20150327

Anti-Tenascin C Antibody

PA2055

Polyclonal

Rabbit IgG polyclonal antibody for Tenascin(TNC) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, rat Predicted to work with: mouse

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Concentration: 0.5-1μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the C-terminus of mouse Tenascin C(2021-2036aa DSMNYHNGRSFSTYDK), identical to the related rat sequence, and different from the related human sequence by two amino acids.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

TNC(Tenascin C), also known as TN or HXB, is a protein that in humans is encoded by the TNC gene. By means of Southern analysis of rodent-human somatic cell hybrids and by in situ hybridization using hexabrachion cDNA probes, Gulcher et al.(1990) localized the TNC gene to chromosome 9q32-q34. Olson and Srivastava(1996) reviewed processes involved in separation of the cardiac tube into the atria, ventricles, and outflow tract. They noted that tenascin is upregulated and NCAM is downregulated in the embryonic endocardial cushions. Gherzi et al.(1997) found that the TNC gene is subject to transcriptional control by OTX2.

1. Gherzi, R., Briata, P., Boncinelli, E., Ponassi, M., Querze, G., Viti, F., Corte, G., Zardi, L. The human homeodomain protein OTX2 binds to the human tenascin-C promoter and trans-represses its activity in transfected cells. DNA Cell Biol. 16: 559-567, 1997. 2. Gulcher, J. R., Alexakos, M. J., Le Beau, M. M., Lemons, R. S., Stefansson, K. Chromosomal localization of the human hexabrachion (tenascin) gene and evidence for recent reduplication within the gene. Genomics 6: 616-622, 1990. 3. Olson, E., Srivastava, D. Molecular pathways controlling heart development. Science 272: 671-676, 1996.

TNC

Tenascin

tenascin C

150 225 antibody Cytotactin antibody Glioma associated extracellular matrix antigen antibody Glioma-associated-extracellular matrix antigen antibody GMEM antibody GP 150 225 antibody GP 150-225 antibody GP antibody Hexabrachion antibody HXB antibody JI antibody MGC167029 antibody Miotendinous antigen antibody Myotendinous antigen antibody Neuronectin antibody TENA_HUMAN antibody Tenascin antibody Tenascin-C antibody Tenascin-C isoform 14/AD1/16 antibody TenascinC antibody TN antibody TN C antibody TN-C antibody TNC antibody

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