Anti-RIP Antibody | Boster Immunoleader PA2051 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-RIP Antibody

catalog :

PA2051

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human

application :

western blot

publications citing this reagent :

Chen Y, Zhao Z, Wu Z, Zou Z, Luo X, Li J, et al. The role of RIP1 and RIP3 in the development of aplastic anemia induced by cyclophosphamide and busulphan in mice. Int J Clin Exp Pathol. 2014;7:8411-20 pubmed

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-RIP Antibody

Catalog Number :

PA2051

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Receptor-interacting serine/threonine-protein kinase 1(RIPK1) detection. Tested with WB in Human.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human

Applications :

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Notes :

WB: The detection limit for RIPK1 is approximately 1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence in the middle region of human RIP(411-425aa RRRRVSHDPFAQQRP).

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Background :

RIPK1(Regulator of G Protein Signaling 3), also called RIP, is an enzyme that in humans is encoded by the RIPK1 gene. Members of the TRAF protein family have been implicated in the activation of NF-kappa-B by the TNF superfamily. By yeast 2-hybrid and coimmunoprecipitation studies using mammalian cell extracts, Hsu et al.(1996) showed that RIP interacts with TRADD, TRAF1, TRAF2, and TRAF3. Hartz(2012) mapped the RIPK1 gene to chromosome 6p25.2 based on an alignment of the RIPK1 sequence with the genomic sequence. Stanger et al.(1995) found that overexpression of Rip in mammalian cells induced morphologic changes characteristic of apoptosis. They suggested that RIP may be an important element in the signal transduction machinery that mediates programmed cell death.

Reference :

1. Hartz, P. A. Personal Communication. Baltimore, Md. 10/26/2012. 2. Hsu, H., Huang, J., Shu, H.-B., Baichwal, V., Goeddel, D. V. TNF-dependent recruitment of the protein kinase RIP to the TNF receptor-1 signaling complex. Immunity 4: 387-396, 1996. 3. Stanger, B. Z., Leder, P., Lee, T.-H., Kim, E., Seed, B. RIP: a novel protein containing a death domain that interacts with Fas/APO-1 (CD95) in yeast and causes cell death. Cell 81: 513-523, 1995.

Gene Name :

RIPK1

Protein Name :

Receptor-interacting serine/threonine-protein kinase 1

Gene Full Name :

receptor(TNFRSF)-interacting serine-threonine kinase 1

Synonyms :

Cell death protein RIP antibody FLJ39204 antibody OTTHUMP00000039163 antibody Receptor(TNFRSF) interacting serine threonine kinase 1 antibody receptor interacting protein 1 antibody Receptor interacting protein antibody Receptor interacting serine threonine protein kinase 1 antibody Receptor TNFRSF interacting serine threonine kinase 1 antibody Receptor-interacting protein 1 antibody Receptor-interacting serine/threonine-protein kinase 1 antibody Rinp antibody RIP 1 antibody RIP antibody Rip-1 antibody RIP1 antibody RIPK 1 antibody RIPK1 antibody RIPK1_HUMAN antibody Serine threonine protein kinase RIP antibody Serine/threonine-protein kinase RIP antibody

Uniprot ID :

Q13546

more info or order :

Boster Immunoleader product webpage