Boster Immunoleader

antibody

Anti-Phospholipase A2 Antibody

PA2047

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse, rat

Anti-Phospholipase A2 Antibody

PA2047

Polyclonal

Rabbit IgG polyclonal antibody for Cytosolic phospholipase A2(PLA2G4A) detection. Tested with WB in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, rat Predicted to work with: mouse

WB

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

WB: The detection limit for PLA2G4A is approximately 0.5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the C-terminus of human Phospholipase A2(686-700aa PNQAFKRLHDLMHFN), different from the related rat and mouse sequences by one amino acid.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

PLA2G4A(Phospholipase A2, Group IVA), is an enzyme that in humans is encoded by the PLA2G4A gene. Tay et al.(1995) mapped the PLA2G4A gene to rat chromosome 13 by PCR-based intercross genotyping and to human 1q25 by fluorescence in situ hybridization. By site-directed mutagenesis and biochemical analysis of the recombinant protein, Sharp et al.(1994) determined that ser228 participates in the catalytic mechanism of cPLA2 and that both the phospholipase A2 and the lysophospholipase activities are catalyzed by the same active site residue(s). PLA2G4A, the cytosolic phospholipase A2, appears to subserve transmembrane signaling responses to extracellular ligands(Skorecki, 1995).

1. Sharp, J. D., Pickard, R. T., Chiou, X. G., Manetta, J. V., Kovacevic, S., Miller, J. R., Varshavsky, A. D., Roberts, E. F., Strifler, B. A., Brems, D. N., Kramer, R. M. Serine 228 is essential for catalytic activities of 85-kDa cytosolic phospholipase A2. J. Biol. Chem. 269: 23250-23254, 1994. 2. Skorecki, K. L. Personal Communication. Toronto, Canada 4/19/1995. 3. Tay, A., Simon, J. S., Squire, J., Hamel, K., Jacob, H. J., Skorecki, K. Cytosolic phospholipase A2 gene in human and rat: chromosomal localization and polymorphic markers. Genomics 26: 138-141, 1995.

PLA2G4A

Cytosolic phospholipase A2

phospholipase A2, group IVA(cytosolic, calcium-dependent)

Calcium dependent phospholipid binding protein antibody CPLA 2 antibody cPLA2 alpha antibody cPLA2 antibody Cytosolic phospholipase A2 antibody Cytosolic phospholipase A2 group IVA antibody Lysophospholipase antibody MGC126350 antibody PA24A_HUMAN antibody Phosphatidylcholine 2 acylhydrolase antibody Phosphatidylcholine 2-acylhydrolase antibody Phospholipase A2 group 4 A antibody Phospholipase A2 group IVA(cytosolic calcium dependent) antibody Phospholipase A2 group IVA antibody PhospholipaseA2 antibody PLA2G4 antibody pla2g4a antibody

P47712