Boster Immunoleader

antibody

Anti-MBD1 Antibody

PA2041

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse

Anti-MBD1 Antibody

PA2041

Polyclonal

Rabbit IgG polyclonal antibody for Methyl-CpG-binding domain protein 1(MBD1) detection. Tested with WB in Human;Mouse.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human Predicted to work with: mouse

WB

Concentration: 0.1-0.5μg/ml; Tested Species: Human; Predicted Species: Mouse

WB: The detection limit for MBD1 is approximately 2.5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence in the middle region of human MBD1(366-381aa NQKRQKCRWRQCLQFA), identical to the related mouse sequence.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

MBD1(Methyl-CpG-Binding Domain Protein 1), also known as PCM1 or CXXC3, is a protein that in humans is encoded by the MBD1 gene. Using PCR on a hybrid panel and FISH, Hendrich et al.(1999) mapped the MBD1 gene to chromosome 18q21, 2.1 cM distal to MBD2. Using yeast 2-hybrid analysis, reciprocal immunoprecipitation analysis, and protein pull-down assays, Fujita et al.(2003) showed that MBD1 interacted directly with MCAF. Deletion analysis revealed that the C-terminal transcriptional repressor domain(TRD) of MBD1 interacted with a conserved C-terminal domain of MCAF. Reporter gene assays showed that MCAF increased the repressive function of the isolated TRD of MBD1 against SP1. Chromatin immunoprecipitation analysis revealed that MBD1 linked MCAF to methylated promoters. Uchimura et al.(2006) found that MBD1 was multiply sumoylated in HeLa cells. Sumoylation did not alter the intracellular localization of MBD1 at nuclear foci in C-33A human cervical cancer cells.

1. Fujita, N., Watanabe, S., Ichimura, T., Ohkuma, Y., Chiba, T., Saya, H., Nakao, M. MCAF mediates MBD1-dependent transcriptional repression. Molec. Cell. Biol. 23: 2834-2843, 2003. 2. Hendrich, B., Abbott, C., McQueen, H., Chambers, D., Cross, S., Bird, A. Genomic structure and chromosomal mapping of the murine and human Mbd1, Mbd2, Mbd3, and Mbd4 genes. Mammalian Genome 10: 906-912, 1999. 3. Uchimura, Y., Ichimura, T., Uwada, J., Tachibana, T., Sugahara, S., Nakao, M., Saitoh, H. Involvement of SUMO modification in MBD1- and MCAF1-mediated heterochromatin formation. J. Biol. Chem. 281: 23180-23190, 2006.

MBD1

Methyl-CpG-binding domain protein 1

methyl-CpG binding domain protein 1

CXXC 3 antibody CXXC-type zinc finger protein 3 antibody CXXC3 antibody MBD 1 antibody MBD1 antibody MBD1_HUMAN antibody MECP1 COMPLEX antibody Methyl CpG binding domain protein 1 antibody Methyl CpG binding domain protein 1 isoform PCM1 antibody Methyl CpG binding protein MBD1 antibody Methyl CpG binding protein splice variant 1 antibody Methyl CpG binding protein splice variant 2 antibody Methyl CpG binding protein splice variant 3 antibody Methyl CpG binding protein splice variant 4 antibody Methyl-CpG-binding domain protein 1 antibody Methyl-CpG-binding protein MBD1 antibody PCM 1 antibody PCM1 antibody Protein containing methyl CpG binding domain 1 antibody Protein containing methyl-CpG-binding domain 1 antibody Regulator of fibroblast growth factor 2(FGF 2) transcription antibody RFT antibody The regulator of fibroblast growth factor 2(FGF 2) transcription antibody

Q9UIS9#Q9UIS9-9