Anti-Hepsin Antibody | Boster Immunoleader PA2035 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Hepsin Antibody

catalog :

PA2035

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, rat

application :

western blot

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Hepsin Antibody

Catalog Number :

PA2035

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Serine protease hepsin(HPN) detection. Tested with WB in Human;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, rat

Applications :

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human Hepsin(392-410aa KVSDFREWIFQAIKTHSEA), different from the related mouse sequence by two amino acids, and from the related rat sequence by one amino acid.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Background :

HPN(Hepsin), also known as TMPRSS1, is an enzyme that in humans is encoded by the HPN gene. Tsuji et al.(1991) mapped the hepsin gene to 19q11-q13.2. Using cDNA microarrays, Dhanasekaran et al.(2001) examined gene expression profiles of more than 50 normal and neoplastic prostate specimens and 3 common prostate cancer cell lines. Signature expression profiles of normal adjacent prostate, benign prostatic hypertrophy, localized prostate cancer, and metastatic, hormone-refractory prostate cancer were determined. In 3 different human cell lines, Guipponi et al.(2008) found that TMPRSS1 was stably anchored in the endoplasmic reticulum.

Reference :

1. Dhanasekaran, S. M., Barrette, T. R., Ghosh, D., Shah, R., Varambally, S., Kurachi, K., Pienta, K. J., Rubin, M. A., Chinnaiyan, A. M. Delineation of prognostic biomarkers in prostate cancer. Nature 412: 822-826, 2001. 2. Guipponi, M., Toh, M.-Y., Tan, J., Park, D., Hanson, K., Ballana, E., Kwong, D., Cannon, P. Z. F., Wu, Q., Gout, A., Delorenzi, M., Speed, T. P., Smith, R. J. H., Dahl, H. H., Petersen, M., Teasdale, R. D., Estivill, X., Park, W. J., Scott, H. S. An integrated genetic and functional analysis of the role of type II transmembrane serine proteases (TMPRSSs) in hearing loss. Hum. Mutat. 29: 130-141, 2008. 3. Tsuji, A., Torres-Rosado, A., Arai, T., Le Beau, M. M., Lemons, R. S., Chou, S.-H., Kurachi, K. Hepsin, a cell membrane-associated protease: characterization, tissue distribution, and gene localization. J. Biol. Chem. 266: 16948-16953, 1991.

Gene Name :

HPN

Protein Name :

Serine protease hepsin

Gene Full Name :

hepsin

Synonyms :

EC 3.4.21. antibody HEPS antibody HEPS_HUMAN antibody Hepsin antibody HPN antibody Serine protease hepsin antibody Serine protease hepsin catalytic chain antibody Serine protease hepsin non catalytic chain antibody TMPRSS 1 antibody TMPRSS1 antibody Transmembrane protease serine 1 antibody

Uniprot ID :

P05981

more info or order :

Boster Immunoleader product webpage