Anti-Eif3b Antibody | Boster Immunoleader PA2030 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Eif3b Antibody

catalog :

PA2030

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Eif3b Antibody

Catalog Number :

PA2030

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Eukaryotic translation initiation factor 3 subunit B(EIF3B) detection. Tested with WB in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, rat Predicted to work with: mouse

Applications :

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Notes :

WB: The detection limit for EIF3B is approximately 2.5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human eIF3B(755-775aa EDFRKYRKMAQELYMEQKNER), different from the related rat and mouse sequences by one amino acid.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Background :

EIF3B(Eukaryotic Translation Initiation Factor 3, Subunit B), also called PRT1 or EIF3-p116, is a protein that in humans is encoded by the EIF3B gene. By searching an EST database, Methot et al.(1997) identified a cDNA encoding a human Prt1 homolog. Asano et al.(1997) demonstrated that the 115-kD component of HeLa cell eIF3 is actually composed of 2 proteins, p116(PRT1) and an unrelated 110-kD protein. Chaudhuri et al.(1997) isolated cDNAs encoding PRT1, which they called p110 based on the size of the corresponding component of rabbit eIF3.

Reference :

1. Asano, K., Kinzy, T. G., Merrick, W. C., Hershey, J. W. B. Conservation and diversity of eukaryotic translation initiation factor eIF3. J. Biol. Chem. 272: 1101-1109, 1997. 2. Chaudhuri, J., Chakrabarti, A., Maitra, U. Biochemical characterization of mammalian translation initiation factor 3 (eIF3): molecular cloning reveals that p110 subunit is the mammalian homologue of saccharomyces cerevisiae protein Prt1. J. Biol. Chem. 272: 30975-30983, 1997. 3. Methot, N., Rom, E., Olsen, H., Sonenberg, N. The human homologue of the yeast Prt1 protein is an integral part of the eukaryotic initiation factor 3 complex and interacts with p170. J. Biol. Chem. 272: 1110-1116, 1997.

Gene Name :

EIF3B

Protein Name :

Eukaryotic translation initiation factor 3 subunit B

Gene Full Name :

eukaryotic translation initiation factor 3, subunit B

Synonyms :

eIF-3-eta antibody eIF3 eta antibody eIF3 p110 antibody eIF3 p116 antibody EIF3-ETA antibody EIF3-P110 antibody EIF3-P116 antibody eIF3b antibody EIF3B_HUMAN antibody EIF3S9 antibody Eukaryotic translation initiation factor 3, subunit B antibody Eukaryotic translation initiation factor 3 subunit 9 antibody eukaryotic translation initiation factor 3 subunit 9 eta antibody Eukaryotic translation initiation factor 3 subunit B antibody hPrt1 antibody PRT1 antibody Prt1 homolog antibody

Uniprot ID :

P55884

more info or order :

Boster Immunoleader product webpage