Boster Immunoleader

antibody

Anti-EIF2S1 Antibody

PA2029

100 ug/vial

200 USD

polyclonal

rabbit

western blot, immunohistochemistry - paraffin section

Anti-EIF2S1 Antibody

PA2029

Polyclonal

Rabbit IgG polyclonal antibody for Eukaryotic translation initiation factor 2 subunit 1(EIF2S1) detection. Tested with WB, IHC-P in Human.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Concentration: 0.5-1μg/ml; Tested Species: Human

WB: The detection limit for EIF2S1 is approximately 0.5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence in the middle region of human EIF2S1(123-137aa KDEQLESLFQRTAWV).

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

EIF2S1(Eukaryotic Translation Initiation Factor 2, Subunit 1), also called EIF2-alpha, is a protein that in humans is encoded by the EIF2S1 gene. Hartz(2010) mapped the EIF2S1 gene to chromosome 14q23.3 based on an alignment of the EIF2S1 sequence with the genomic sequence(GRCh37). Ernst et al.(1987) stated that protein synthesis is inhibited due to phosphorylation of Eif2-alpha in hemin-deprived rabbit reticulocyte lysates. HeLa cells subjected to heat shock, serum deprivation, or interferon treatment followed by virus infection also show a correlation between EIF2-alpha phosphorylation and translational repression. Jacob et al.(1989) found that the alpha-Pal transcription factor bound to 2 palindromic sites within the EIF2-alpha promoter and was essential for transcription of the EIF2-alpha gene.

1. Ernst, H., Duncan, R. F., Hershey, J. W. B. Cloning and sequencing of complementary DNAs encoding the alpha-subunit of translational initiation factor eIF-2: characterization of the protein and its messenger RNA. J. Biol. Chem. 262: 1206-1212, 1987. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 1/28/2010. 3. Jacob, W. F., Silverman, T. A., Cohen, R. B., Safer, B. Identification and characterization of a novel transcription factor participating in the expression of eukaryotic initiation factor 2 alpha. J. Biol. Chem. 264: 20372-20384, 1989.

EIF2S1

Eukaryotic translation initiation factor 2 subunit 1

eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa

EIF 2 alpha antibody EIF 2 antibody EIF 2A antibody EIF 2alpha antibody eIF-2-alpha antibody eIF-2A antibody EIF-2alpha antibody EIF2 alpha antibody EIF2 antibody EIF2A antibody EIF2S1 antibody Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa antibody Eukaryotic translation initiation factor 2 subunit 1 alpha antibody Eukaryotic translation initiation factor 2 subunit 1 antibody Eukaryotic translation initiation factor 2 subunit alpha antibody IF2A_HUMAN antibody

P05198