Anti-PERK Antibody | Boster Immunoleader PA2026 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-PERK Antibody

catalog :

PA2026

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human

application :

western blot, immunocytochemistry

publications citing this reagent :

Wang Y, Tian F, Liu H, Zhang W, Li J, Xiao B, et al. Macrophage migration inhibitory factor is necessary for the lipo-oligosaccharide-induced response by modulation of Toll-like receptor 4 in monocytes from GBS patients. J Neuroimmunol. 2013;257:67-75 pubmed publisher

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-PERK Antibody

Catalog Number :

PA2026

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Eukaryotic translation initiation factor 2-alpha kinase 3(EIF2AK3) detection. Tested with WB, ICC in Human.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human

Applications :

WB,ICC

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Immunocytochemistry :

Concentration: 0.5-1μg/ml; Tested Species: Human

Notes :

WB: The detection limit for EIF2AK3 is approximately 0.5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the N-terminus of human PERK(163-176aa QWDQDRESMETVPF).

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in ICC.

Background :

EIF2AK3(Eukaryotic Translation Initiation Factor 2-Alpha Kinase 3), also called PEK, is an enzyme that in humans is encoded by the EIF2AK3 gene. By fluorescence in situ hybridization and radiation hybrid analysis, Hayes et al.(1999) mapped the EIF2AK3 gene to chromosome 2p12. Harding et al.(2000) reported that a targeted mutation of the mouse Eif2ak3 gene, which they called Perk, abolished the phosphorylation of eIF2-alpha in response to accumulation of malfolded proteins in the ER, resulting in abnormally elevated protein synthesis and higher levels of ER stress. Using a library of endoribonuclease-prepared short interfering RNAs(esiRNAs), Kittler et al.(2004) identified 37 genes required for cell division, one of which was EIF2AK3.

Reference :

1. Harding, H. P., Zhang, Y., Bertolotti, A., Zeng, H., Ron, D. Perk is essential for translational regulation and cell survival during the unfolded protein response. Molec. Cell 5: 897-904, 2000. 2. Hayes, S. E., Conner, L. J., Stramm, L. E., Shi, Y. Assignment of pancreatic eIF-2a kinase (EIF2AK3) to human chromosome band 2p12 by radiation hybrid mapping and in situ hybridization. Cytogenet. Cell Genet. 86: 327-328, 1999. 3. Kittler, R., Putz, G., Pelletier, L., Poser, I., Heninger, A.-K., Drechsel, D., Fischer, S., Konstantinova, I., Habermann, B., Grabner, H., Yaspo, M.-L., Himmelbauer, H., Korn, B., Neugebauer, K., Pisabarro, M. T., Buchholz, F. An endoribonuclease-prepared siRNA screen in human cells identifies genes essential for cell division. Nature 432: 1036-1040, 2004.

Gene Name :

EIF2AK3

Protein Name :

Eukaryotic translation initiation factor 2-alpha kinase 3

Gene Full Name :

eukaryotic translation initiation factor 2-alpha kinase 3

Synonyms :

DKFZp781H1925 antibody E2AK3_HUMAN antibody EC 2.7.11.1 antibody EIF2AK3 antibody Eukaryotic translation initiation factor 2 alpha kinase 3 antibody Eukaryotic translation initiation factor 2-alpha kinase 3 antibody Heme regulated EIF2 alpha kinase antibody HRI antibody HsPEK antibody Pancreatic eIF2 alpha kinase antibody Pancreatic eIF2-alpha kinase antibody PEK antibody PRKR like endoplasmic reticulum kinase antibody PRKR-like endoplasmic reticulum kinase antibody WRS antibody

Uniprot ID :

Q9NZJ5

more info or order :

Boster Immunoleader product webpage