Boster Immunoleader

antibody

Anti-DISC1 Antibody

PA2023-1

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse

western blot, immunohistochemistry - paraffin section

info changed 20150327

Anti-DISC1 Antibody

PA2023-1

Polyclonal

Rabbit IgG polyclonal antibody for Disrupted in schizophrenia 1 protein(DISC1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, mouse, rat

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Concentration: 0.5-1μg/ml; Tested Species: Human, Mouse, Rat

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence in the middle region of human DISC1(461-477aa EKQQLQKEIEALQARMF), different from the related mouse sequence by two amino acids, and from the related rat sequence by four amino acids.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

DISC1(Disrupted in Schizophrenia 1), is a protein that is encoded by the DISC1 gene in humans. Ma et al.(2002) determined that the mouse Disc1 gene maps to chromosome 8 in a region with homology of synteny to human chromosome 1q42. Ozeki et al.(2003) demonstrated that rodent Disc1 expression displayed pronounced developmental regulation, with the highest levels in late embryonic life during development of the cerebral cortex. Millar et al.(2005) showed that DISC1 interacts with the UCR2 domain of phosphodiesterase-4B, implicated in susceptibility to schizophrenia, and that elevation of cellular cAMP leads to dissociation of PDE4B from DISC1 and in increase in PDE4B activity.

1. Ma, L., Liu, Y., Ky, B., Shughrue, P. J., Austin, C. P., Morris, J. A. Cloning and characterization of Disc1, the mouse ortholog of DISC1 (disrupted-in-schizophrenia 1). Genomics 80: 662-672, 2002. 2. Millar, J. K., Pickard, B. S., Mackie, S., James, R., Christie, S., Buchanan, S. R., Malloy, M. P., Chubb, J. E., Huston, E., Baillie, G. S., Thomson, P. A., Hill, E. V., Brandon, N. J., Rain, J.-C., Camargo, L. M., Whiting, P. J., Houslay, M. D., Blackwood, D. H. R., Muir, W. J., Porteous, D. J. DISC1 and PDE4B are interacting genetic factors in schizophrenia that regulate cAMP signaling. Science 310: 1187-1191, 2005. 3. Ozeki, Y., Tomoda, T., Kleiderlein, J., Kamiya, A., Bord, L., Fujii, K., Okawa, M., Yamada, N., Hatten, M. E., Snyder, S. H., Ross, C. A., Sawa, A. Disrupted-in-schizophrenia-1 (DISC-1): mutant truncation prevents binding to NudE-like (NUDEL) and inhibits neurite outgrowth. Proc. Nat. Acad. Sci. 100: 289-294, 2003.

DISC1

Disrupted in schizophrenia 1 protein

disrupted in schizophrenia 1

C1orf136 antibody DISC1 antibody DISC1_HUMAN antibody Disrupted in schizophrenia 1 antibody Disrupted in schizophrenia 1 protein antibody KIAA0457 antibody RP4-730B13.1 antibody SCZD9 antibody

Q9NRI5