Anti-CD68 Antibody | Boster Immunoleader PA2018 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-CD68 Antibody

catalog :

PA2018

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

publications citing this reagent :

Peng K, Liu L, Wei D, Lv Y, Wang G, Xiong W, et al. P2X7R is involved in the progression of atherosclerosis by promoting NLRP3 inflammasome activation. Int J Mol Med. 2015;35:1179-88 pubmed publisher
Hao L, Wang X, Cheng J, You S, Ma S, Zhong X, et al. The up-regulation of endothelin-1 and down-regulation of miRNA-125a-5p, -155, and -199a/b-3p in human atherosclerotic coronary artery. Cardiovasc Pathol. 2014;23:217-23 pubmed publisher
Chen X, Wan J, Liu J, Xie W, Diao X, Xu J, et al. Increased IL-17-producing cells correlate with poor survival and lymphangiogenesis in NSCLC patients. Lung Cancer. 2010;69:348-54 pubmed publisher

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-CD68 Antibody

Catalog Number :

PA2018

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Macrosialin(CD68) detection. Tested with WB, IHC-P in Human.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human

Notes :

WB: The detection limit for CD68 is approximately 1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human CD68(341-354aa FCIIRRRPSAYQAL).

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

CD68, cluster of differentiation, is a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. CD68 is a member of a family of hematopoietic mucin-like molecules that includes leukosialin/CD43 and stem cell antigen CD34. The CD68 gene is mapped to 17p13.1. Immunohistochemistry can be used to identify the presence of CD68, which is found in the cytoplasmic granules of a range of different blood cells. It is particularly useful as a marker for the various cells of the macrophage lineage, including monocytes, histiocytes, giant cells, Kupffer cells, and osteoclasts. This allows it to be used to distinguish diseases of otherwise similar appearance, such as the monocyte/macrophage and lymphoid forms of leukaemia(the latter being CD68 negative). Its presence in macrophages also makes it useful in diagnosing conditions related to proliferation or abnormality of these cells, such as malignant histiocytosis, histiocytic lymphoma, and Gaucher's disease.

Reference :

1. Holness, C. L., Simmons, D. L.Molecular cloning of CD68, a human macrophage marker related to lysosomal glycoproteins.Blood 81: 1607-1613, 1993. 2. Jones, E., Quinn, C. M., See, C. G., Montgomery, D. S., Ford, M. J., Kolble, K., Gordon, S., Greaves, D. R.The linked human elongation initiation factor 4A1 (EIF4A1) and CD68 genes map to chromosome 17p13.Genomics 53: 248-250, 1998.

Gene Name :

CD68

Protein Name :

Macrosialin

Gene Full Name :

CD68 molecule

Synonyms :

CD 68 antibody CD68 antibody CD68 antigen antibody CD68 molecule antibody CD68_HUMAN antibody DKFZp686M18236 antibody GP110 antibody LAMP4 antibody Macrophage antigen CD68(microsialin) antibody MACROPHAGE ANTIGEN CD68 antibody macrosialin antibody SCARD1 antibody Scavenger receptor class D member 1 antibody

Uniprot ID :

P34810

more info or order :

Boster Immunoleader product webpage