Anti-Adiponectin Antibody | Boster Immunoleader PA2014-1 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Adiponectin Antibody

catalog :

PA2014-1

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

publications citing this reagent :

Liu L, Gu H, Zhao Y, An L, Yang J. Glypican 4 may be involved in the adipose tissue redistribution in high-fat feeding C57BL/6J mice with peroxisome proliferators-activated receptor γ agonist rosiglitazone treatment. Exp Ther Med. 2014;8:1813-1818 pubmed

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Boster Immunoleader product webpage

product information

Product Name :

Anti-Adiponectin Antibody

Catalog Number :

PA2014-1

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Adiponectin(ADIPOQ) detection. Tested with WB, IHC-P, IHC-F, ELISA in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, mouse, rat

Applications :

WB,IHC-P,IHC-F,ELISA

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human, Mouse, Rat

Immunohistochemistry(Frozen Section) :

Concentration: 0.5-1μg/ml; Tested Species: Rat; Predicted Species: Human, Mouse

ELISA :

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Notes :

WB: The detection limit for ADIPOQ is approximately 1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human ADIPOQ(224-244aa LYADNDNDSTFTGFLLYHDTN), different from the related mouse and rat sequences by one amino acid.

Contents :

Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P) and IHC(F).

Background :

ADIPOQ(Adipocyte-, C1q-, and Collagen Domain-Containing), also known as APM1, ADPN or ACDC, is a protein which in humans is encoded by the ADIPOQ gene. Using FISH, Das et al.(2001) mapped the mouse Acrp30 gene to chromosome 16 in a region showing homology of synteny with human 3q27. By RNase protection and Western blot analysis, Schaffler et al.(1999) showed that APM1 is expressed by differentiated adipocytes as a 33-kD protein that is also detectable in serum. By sequence comparisons, they found links between APM1 and TNF family ligands as well as to cytokines expressed by T cells. Adiponectin is a protein hormone that modulates a number of metabolic processes, including glucose regulation and fatty acid oxidation. Adiponectin is exclusively secreted from adipose tissue(and also from the placenta in pregnancy) into the bloodstream and is very abundant in plasma relative to many hormones.

Reference :

1. Das, K., Lin, Y., Widen, E., Zhang, Y., Scherer, P. E. Chromosomal localization, expression pattern, and promoter analysis of the mouse gene encoding adipocyte-specific secretory protein Acrp30. Biochem. Biophys. Res. Commun. 280: 1120-1129, 2001. 2. Schaffler, A., Orso, E., Palitzsch, K.-D., Buchler, C., Drobnik, W., Furst, A., Scholmerich, J., Schmitz, G. The human apM-1, an adipocyte-specific gene linked to the family of TNF's and to genes expressed in activated T cells, is mapped to chromosome 1q21.3-q23, a susceptibility locus identified for familial combined hyperlipidaemia (FCH). Biochem. Biophys. Res. Commun. 260: 416-425, 1999.

Gene Name :

ADIPOQ

Protein Name :

Adiponectin

Gene Full Name :

adiponectin, C1Q and collagen domain containing

Synonyms :

30 kDa adipocyte complement related protein antibody 30 kDa adipocyte complement-related protein antibody ACDC antibody ACRP 30 antibody ACRP30 antibody ADIPO_HUMAN antibody Adipocyte antibody Adipocyte C1q and collagen domain containing protein antibody Adipocyte complement related 30 kDa protein antibody Adipocyte complement related protein of 30 kDa antibody Adipocyte complement-related 30 kDa protein antibody Adiponectin antibody AdipoQ antibody Adipose most abundant gene transcript 1 antibody Adipose most abundant gene transcript 1 protein antibody Adipose specific collagen like factor antibody ADIPQTL1 antibody ADPN antibody APM 1 antibody apM-1 antibody ApM1 antibody C1q and collagen domain-containing protein antibody GBP 28 antibody GBP28 antibody Gelatin binding protein antibody Gelatin binding protein 28 antibody Gelatin-binding protein antibody

Uniprot ID :

Q15848

more info or order :

Boster Immunoleader product webpage