Anti-T-bet/Tbx21 Antibody | Boster Immunoleader PA1999 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-T-bet/Tbx21 Antibody

catalog :

PA1999

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot

publications citing this reagent :

Miao L, Run-Ming J, Yi J. T-Bet Mediated Anti-Neoplastic Effects of Dendritic Cell-Cytokine Induced Killer Cells in vitro. Iran J Pediatr. 2012;22:43-51 pubmed

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-T-bet/Tbx21 Antibody

Catalog Number :

PA1999

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for T-box transcription factor TBX21(TBX21) detection. Tested with WB in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, rat Predicted to work with: mouse

Applications :

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Notes :

WB: The detection limit for TBX21 is approximately 0.5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human T-bet(522-535aa DKEAEGQFYNYFPN), different from the related rat and mouse sequences by one amino acid.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Background :

TBX21(T-Box 21), also called TBET, is a protein that in humans is encoded by the TBX21 gene. This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. Zhang and Yang(2000) mapped the mouse Tbx21 gene to chromosome 11D in an area showing homology of synteny with human chromosome 17. Szabo et al.(2000) showed that TBX21 expression correlates with IFNG expression in Th1 and natural killer(NK) cells. Ectopic expression of TBX21 both transactivated the IFNG gene and induced endogenous IFNG production. Finotto et al.(2002) observed reduced expression of the T(H)1 transcription factor Tbet in T cells from airways of patients with asthma compared with that in T cells from airways of nonasthmatic patients, suggesting that loss of Tbet might be associated with asthma.

Reference :

1. Finotto, S., Neurath, M. F., Glickman, J. N., Qin, S., Lehr, H. A., Green, F. H. Y., Ackerman, K., Haley, K., Galle, P. R., Szabo, S. J., Drazen, J. M., De Sanctis, G. T., Glimcher, L. H. Development of spontaneous airway changes consistent with human asthma in mice lacking T-bet. Science 295: 336-338, 2002. 2. Szabo, S. J., Kim, S. T., Costa, G. L., Zhang, X., Fathman, C. G., Glimcher, L. H. A novel transcription factor, T-bet, directs Th1 lineage commitment. Cell 100: 655-669, 2000. 3. Zhang, W., Yang, S. Y. Cloning and characterization of a new member of the T-box gene family. Genomics 70: 41-48, 2000.

Gene Name :

TBX21

Protein Name :

T-box transcription factor TBX21

Gene Full Name :

T-box 21

Synonyms :

T bet antibody T box 21 antibody T box expressed in T cells antibody T box protein 21 antibody T box transcription factor TBX21 antibody T cell specific T box transcription factor antibody T cell specific T box transcription factor T bet antibody T PET antibody T-box protein 21 antibody T-box transcription factor TBX21 antibody T-cell-specific T-box transcription factor T-bet antibody TBET antibody TBLYM antibody TBX 21 antibody TBX21 antibody TBX21_HUMAN antibody TPET antibody Transcription factor TBLYM antibody

Uniprot ID :

Q9UL17

more info or order :

Boster Immunoleader product webpage