Boster Immunoleader

antibody

Anti-SPHK1 Antibody

PA1996

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse

western blot, immunocytochemistry, immunohistochemistry - paraffin section

Anti-SPHK1 Antibody

PA1996

Polyclonal

Rabbit IgG polyclonal antibody for Sphingosine kinase 1(SPHK1) detection. Tested with WB, IHC-P, ICC in Human;Mouse.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human Predicted to work with: mouse

WB,IHC-P,ICC

Concentration: 0.1-0.5μg/ml; Tested Species: Human; Predicted Species: Rat

Concentration: 0.5-1μg/ml; Tested Species: Human; Predicted Species: Mouse

Concentration: 0.5-1μg/ml; Tested Species: Human

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence in the middle region of human SPHK1(117-133aa AASLNHYAGYEQVTNED), different from the related rat sequence by two amino acids, and from the related mouse sequence by one amino acid.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P) and ICC.

SPHK1(Sphingosine Kinase 1), is an enzyme that in humans is encoded by the SPHK1 gene. Melendez et al.(2000) mapped the SPHK1 gene to chromosome 17q25.2 based on sequence identity with ESTs mapped to this region. Kohama et al.(1998) demonstrated that recombinant mouse Sphk1 can specifically phosphorylate D-erythro-sphingosine and that D, L-threo-dihydrosphingosine and N, N-dimethylsphingosine can act as competitive inhibitors of recombinant Sphk1. Pitson et al.(2000) found that recombinant SPHK1 and endogenous SPHK1 purified from placenta had identical enzymatic characteristics, suggesting posttranslational modification does not effect functional properties.

1. Kohama, T., Olivera, A., Edsall, L., Nagiec, M. M., Dickson, R., Spiegel, S. Molecular cloning and functional characterization of murine sphingosine kinase. J. Biol. Chem. 273: 23722-23728, 1998. 2. Melendez, A. J., Carlos-Dias, E., Gosink, M., Allen, J. M., Takacs, L. Human sphingosine kinase: molecular cloning, functional characterization and tissue distribution. Gene 251: 19-26, 2000. 3. Pitson, S. M., D'Andrea, R. J., Vandeleur, L., Moretti, P. A. B., Xia, P., Gamble, J. R., Vadas, M. A., Wattenberg, B. W. Human sphingosine kinase: purification, molecular cloning and characterization of the native and recombinant enzymes. Biochem. J. 350: 429-441, 2000.

SPHK1

Sphingosine kinase 1

sphingosine kinase 1

SK 1 antibody SK1 antibody Sphingosine Kinase 1 antibody SPHK 1 antibody SPHK antibody Sphk1 antibody SPHK1_HUMAN antibody SPK 1 antibody SPK antibody SPK1 antibody

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