Anti-UCP1 Antibody | Boster Immunoleader PA1981 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-UCP1 Antibody

catalog :

PA1981

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human

application :

western blot

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-UCP1 Antibody

Catalog Number :

PA1981

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Mitochondrial brown fat uncoupling protein 1(UCP1) detection. Tested with WB in Human.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human

Applications :

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Notes :

WB: The detection limit for UCP1 is approximately 0.5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human UCP1(289-307aa FEQLKRELSKSRQTMDCAT).

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Background :

UCP1(Uncoupling Protein 1), also called THERMOGENIN or UCP, is an uncoupling protein found in the mitochondria of brown adipose tissue(BAT). Using in situ hybridization, Cassard et al.(1990) assigned the human UCP gene to 4q31. They found that the primary structure of UCP is similar to that of ADP/ATP translocator of skeletal muscle, the gene for which is also located on chromosome 4. The failure of UCP1 expressed in E. coli inclusion bodies to carry out fatty acid-dependent H+ transport activity inclusion bodies made Echtay et al.(2000) seek a native UCP cofactor. They identified coenzyme Q(CoQ, or ubiquinone) as such a cofactor. On addition of CoQ(10) to reconstituted UCP1 from inclusion bodies, fatty acid-dependent proton transport reached the same rate as with native UCP1.

Reference :

1. Cassard, A. M., Bouillaud, F., Mattei, M. G., Hentz, E., Raimbault, S., Thomas, M., Ricquier, D. Human uncoupling protein gene: structure, comparison with rat gene, and assignment to the long arm of chromosome 4. J. Cell. Biochem. 43: 255-264, 1990. 2. Echtay, K. S., Winkler, E., Klingenberg, M. Coenzyme Q is an obligatory cofactor for uncoupling protein function. Nature 408: 609-613, 2000.

Gene Name :

UCP1

Protein Name :

Mitochondrial brown fat uncoupling protein 1

Gene Full Name :

uncoupling protein 1(mitochondrial, proton carrier)

Synonyms :

mitochondrial brown fat uncoupling protein antibody Mitochondrial brown fat uncoupling protein 1 antibody SLC25A7 antibody Solute carrier family 25 member 7 antibody Thermogenin antibody UCP 1 antibody UCP antibody UCP1 antibody UCP1_HUMAN antibody uncoupling protein 1(mitochondrial, proton carrier) antibody Uncoupling protein 1 antibody

Uniprot ID :

P25874

more info or order :

Boster Immunoleader product webpage