Anti-53BP1 Antibody | Boster Immunoleader PA1976 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-53BP1 Antibody

catalog :

PA1976

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunohistochemistry - paraffin section

publications citing this reagent :

Chen X, Gong J, Zeng H, Chen N, Huang R, Huang Y, et al. MicroRNA145 targets BNIP3 and suppresses prostate cancer progression. Cancer Res. 2010;70:2728-38 pubmed publisher

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Boster Immunoleader product webpage

product information

Availability :

info changed 20150325

Product Name :

Anti-53BP1 Antibody

Catalog Number :

PA1976

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Tumor suppressor p53-binding protein 1(TP53BP1) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, mouse, rat

Applications :

WB,IHC-P,ICC

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Mouse; Predicted Species: Rat

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human, Mouse, Rat

Immunocytochemistry :

Concentration: 0.5-1μg/ml; Tested Species: Human; Predicted Species: Mouse, Rat

Notes :

WB: The detection limit for TP53BP1 is approximately 2.5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human 53BP1(1955-1972aa ERIGFKQHPKYKHDYVSH), identical to the related mouse sequence.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P) and ICC.

Background :

TP53BP1(Tumor Protein p53-Binding Protein 1), also called 53BP1, is a protein that in humans is encoded by the TP53BP1 gene. Iwabuchi et al.(1998) mapped the TP53BP1 gene to 15q15-q21 by FISH. Iwabuchi et al.(1994) showed that TP53BP1 binds to the conformationally sensitive central domain of wildtype p53 but not to mutant p53 in vitro. Immunoblot analysis by Iwabuchi et al.(1998) showed that expression of TP53BP1 or TP53BP2 enhances the transactivation function of p53 and induces the expression of p21(CDKN1A). Wang et al.(2002) used small interfering RNA directed against TP53BP1 in mammalian cells to demonstrate that TP53BP1 is a key transducer of the DNA damage checkpoint signal. TP53BP1 was required for p53 accumulation, G2/M checkpoint arrest, and the intra-S-phase checkpoint in response to ionizing radiation.

Reference :

1. Iwabuchi, K., Bartel, P. L., Li, B., Marraccino, R., Fields, S. Two cellular proteins that bind to wild-type but not mutant p53. Proc. Nat. Acad. Sci. 91: 6098-6102, 1994. 2. Iwabuchi, K., Li, B., Massa, H. F., Trask, B. J., Date, T., Fields, S. Stimulation of p53-mediated transcriptional activation by the p53-binding proteins, 53BP1 and 53BP2. J. Biol. Chem. 273: 26061-26068, 1998 3. Wang, B., Matsuoka, S., Carpenter, P. B., Elledge, S. J. 53BP1, a mediator of the DNA damage checkpoint. Science 298: 1435-1438, 2002.

Gene Name :

TP53BP1

Protein Name :

Tumor suppressor p53-binding protein 1

Gene Full Name :

tumor protein p53 binding protein 1

Synonyms :

53 BP1 antibody 53BP1 antibody FLJ41424 antibody MGC138366 antibody p202 antibody p53 binding protein 1 antibody p53 BP1 antibody p53-binding protein 1 antibody p53BP1 antibody TP53 BP1 antibody TP53B_HUMAN antibody TP53BP1 antibody TRP53 BP1 antibody Tumor protein 53 binding protein 1 antibody Tumor protein p53 binding protein 1 antibody Tumor suppressor p53 binding protein 1 antibody Tumor suppressor p53-binding protein 1 antibody

Uniprot ID :

Q12888

more info or order :

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