Anti-DNA PKcs Antibody | Boster Immunoleader PA1970 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-DNA PKcs Antibody

catalog :

PA1970

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human

application :

western blot

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-DNA PKcs Antibody

Catalog Number :

PA1970

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for DNA-dependent protein kinase catalytic subunit(PRKDC) detection. Tested with WB in Human.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human

Applications :

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Notes :

WB: The detection limit for PRKDC is approximately 2.5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the N-terminus of human DNA PKcs(328-346aa AKNAEMHKNKLQYFMEQFY).

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Background :

PRKDC(Protein Kinase DNA-Activated Catalytic Subunit), also called DNAPK, HYRC1, p350 or DNPK1, is an enzyme that in humans is encoded by the PRKDC gene. DNA-PKcs belongs to the phosphatidylinositol 3-kinase-related kinase protein family. Satoh et al.(1997) mapped the MCM4 gene to 8q11.2 by FISH. Based on the close proximity of the PRKDC and MCM4 genes, it was assumed that the PRKDC gene also maps to this location. Anderson and Lees-Miller(1992) noted that DNA-PK had been shown in vitro to phosphorylate several transcription factors, suggesting that it functions in cell homeostasis by modulating transcription. Daniel et al.(1999) demonstrated that the PRKDC protein participates in retroviral DNA integration, which is catalyzed by the viral protein integrase.

Reference :

1. Anderson, C. W., Lees-Miller, S. P. The nuclear serine/threonine protein kinase DNA-PK. Crit. Rev. Eukaryot. Gene Expr. 2: 283-314, 1992. 2. Daniel, R., Katz, R. A., Skalka, A. M. A role for DNA-PK in retroviral DNA integration. Science 284: 644-647, 1999. 3. Satoh, T., Tsuruga, H., Yabuta, N., Ishidate, M., Jr., Nojima, H. Assignment of the human CDC21 (MCM4) gene to chromosome 8q11.2. Genomics 46: 525-526, 1997.

Gene Name :

PRKDC

Protein Name :

DNA-dependent protein kinase catalytic subunit

Gene Full Name :

protein kinase, DNA-activated, catalytic polypeptide

Synonyms :

DNA dependent protein kinase catalytic subunit antibody DNA PK catalytic subunit antibody DNA-dependent protein kinase catalytic subunit antibody DNA-PK catalytic subunit antibody DNA-PKcs antibody DNAPK antibody DNAPK catalytic subunit antibody DNPK 1 antibody DNPK1 antibody Hyper radiosensitivity of murine scid mutation, complementing 1 antibody HYRC 1 antibody HYRC antibody HYRC1 antibody p350 antibody p460 antibody PKRDC antibody PRKDC antibody PRKDC_HUMAN antibody Protein Kinase DNA Activated Catalytic Polypeptide antibody XRCC 7 antibody XRCC7 antibody

Uniprot ID :

P78527

more info or order :

Boster Immunoleader product webpage