Anti-LCAT Antibody | Boster Immunoleader PA1967 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-LCAT Antibody

catalog :

PA1967

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, rat

application :

western blot

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-LCAT Antibody

Catalog Number :

PA1967

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Phosphatidylcholine-sterol acyltransferase(LCAT) detection. Tested with WB in Human;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, rat

Applications :

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human LCAT(300-313aa RDFQRFFADLHFEE), different from the related rat sequence by two amino acids, and from the mouse sequence by three amino acids.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Background :

LCAT(Lecithin: Cholesterol Acyltransferase), is an enzyme that converts free cholesterol into cholesteryl ester. Azoulay et al.(1987) used a cDNA clone corresponding to LCAT to assign the locus to 16q22 through the analysis of DNA from somatic cell hybrids and in situ hybridization. LCAT plays an important role in lipoprotein metabolism, especially in the process termed "reverse cholesterol transport" The enzyme is synthesized in the liver and circulates in blood plasma as a complex with components of high density lipoprotein(HDL). Cholesterol from peripheral cells is transferred to HDL particles, esterified through the action of LCAT on HDL, and incorporated into the core of the lipoprotein. The cholesterol ester is thereby transported to the liver(Jonas, 2000).

Reference :

1. Azoulay, M., Henry, I., Tata, F., Weil, D., Grzeschik, K. H., Chaves, E., McIntyre, N., Williamson, R., Humphries, S. E., Junien, C. The structural gene for lecithin:cholesterol acyl transferase (LCAT) maps to 16q22. Ann. Hum. Genet. 51: 129-136, 1987. 2. Frohlich, J., McLeod, R., Hon, K. Lecithin:cholesterol acyl transferase (LCAT). Clin. Biochem. 15: 269-278, 1982. 3. Jonas, A. Lecithin cholesterol acyltransferase. Biochim. Biophys. Acta 1529: 245-256, 2000.

Gene Name :

LCAT

Protein Name :

Phosphatidylcholine-sterol acyltransferase

Gene Full Name :

lecithin-cholesterol acyltransferase

Synonyms :

LCAT antibody LCAT_HUMAN antibody Lecithin cholesterol acyltransferase antibody Lecithin-cholesterol acyltransferase antibody Phosphatidylcholine sterol acyltransferase antibody Phosphatidylcholine-sterol acyltransferase antibody Phospholipid cholesterol acyltransferase antibody Phospholipid-cholesterol acyltransferase antibody

Uniprot ID :

P04180

more info or order :

Boster Immunoleader product webpage