Anti-DDX6 Antibody | Boster Immunoleader PA1965 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-DDX6 Antibody

catalog :

PA1965

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

application :

western blot

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-DDX6 Antibody

Catalog Number :

PA1965

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Probable ATP-dependent RNA helicase DDX6(DDX6) detection. Tested with WB in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human Predicted to work with: mouse, rat

Applications :

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human; Predicted Species: Mouse, Rat

Notes :

WB: The detection limit for DDX6 is approximately 0.5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human DDX6(440-453aa YDDRFNLKSIEEQL), identical to the related rat and mouse sequences.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Background :

DDX6(DEAD/H BOX 6), also known as HLR2 or p54, is an enzyme that in humans is encoded by the DDX6 gene. DDX6 belongs to the DEAD box family of putative RNA helicases that contain a characteristic asp-glu-ala-asp(DEAD) box motif(Seto et al., 1995). Tunnacliffe et al.(1993) assigned the DDX6 gene more precisely using a panel of sequence tagged sites(STSs) representing 30 markers previously assigned to 11q23. Using mass spectroscopy, Fenger-Gron et al.(2005) found that RCK, EDC3(YJDC), and HEDLS(RCD8) coimmunopurified with DCP1A and DCP2 from HEK293 cell lysates. Overexpression of DCP2, RCK, or EDC3 in HeLa cells reduced the association of endogenous DCP1A and XRN1 with cytoplasmic P bodies.

Reference :

1. Fenger-Gron, M., Fillman, C., Norrild, B., Lykke-Andersen, J. Multiple processing body factors and the ARE binding protein TTP activate mRNA decapping. Molec. Cell 20: 905-915, 2005. 2. Seto, M., Yamamoto, K., Takahashi, T., Ueda, R. Cloning and expression of a murine cDNA homologous to the human RCK/P54, a lymphoma-linked chromosomal translocation junction gene on 11q23. Gene 166: 293-296, 1995. 3. Tunnacliffe, A., Perry, H., Radice, P., Budarf, M. L., Emanuel, B. S. A panel of sequence tagged sites for chromosome band 11q23. Genomics 17: 744-747, 1993.

Gene Name :

DDX6

Protein Name :

Probable ATP-dependent RNA helicase DDX6

Gene Full Name :

DEAD(Asp-Glu-Ala-Asp) box helicase 6

Synonyms :

ATP dependent RNA helicase DDX6 antibody ATP dependent RNA helicase p54 antibody DDX6 antibody DEAD(Asp Glu Ala Asp) box polypeptide 6 antibody DEAD box 6 antibody DEAD box protein 6 antibody DEAD/H(Asp Glu Ala Asp/His) box polypeptide 6(RNA helicase 54kD) antibody FLJ36338 antibody HLR2 antibody Oncogene RCK antibody P54 antibody Probable ATP-dependent RNA helicase DDX6 antibody RCK antibody

Uniprot ID :

P26196

more info or order :

Boster Immunoleader product webpage