Anti-DDX5 Antibody | Boster Immunoleader PA1964 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-DDX5 Antibody

catalog :

PA1964

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-DDX5 Antibody

Catalog Number :

PA1964

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Probable ATP-dependent RNA helicase DDX5(DDX5) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, mouse, rat

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Mouse, Rat

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Notes :

WB: The detection limit for DDX5 is approximately 2.5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human DDX5(566-580aa TYQNGYDSTQQYGSN), identical to the related rat and mouse sequences.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

DDX5(DEAD/H BOX 5), also known as HLR1 or G17P1, is an enzyme that in humans is encoded by the DDX5 gene. The p68 protein is a proliferation-associated nuclear antigen first identified through its highly specific cross-reaction with the simian virus 40 tumor antigen(Iggo et al., 1989). Subsequently, homology to eukaryotic translation initiation factor was found, and amino acid sequence blocks characteristic of a large superfamily of proteins with putative helicase activity were demonstrated. Brody et al.(1995) confirmed that this gene is located on chromosome 17 in the region of the BRCA1 gene at 17q21. By immunoprecipitation analysis, Caretti et al.(2006) found that p68, p72(DDX17), and the noncoding RNA SRA(SRA1) associated with MYOD(MYOD1) in MYOD-transfected HeLa cells.

Reference :

1. Brody, L. C., Abel, K. J., Castilla, L. H., Couch, F. J., McKinley, D. R., Yin, G.-Y., Ho, P. P., Merajver, S., Chandrasekharappa, S. C., Xu, J., Cole, J. L., Struewing, J. P., Valdes, J. M., Collins, F. S., Weber, B. L. Construction of a transcription map surrounding the BRCA1 locus of human chromosome 17. Genomics 25: 238-247, 1995. 2. Caretti, G., Schiltz, R. L., Dilworth, F. J., Di Padova, M., Zhao, P., Ogryzko, V., Fuller-Pace, F. V., Hoffman, E. P., Tapscott, S. J., Sartorelli, V. The RNA helicases p68/p72 and the noncoding RNA SRA are coregulators of MyoD and skeletal muscle differentiation. Dev. Cell 11: 547-560, 2006. 3. Iggo, R., Gough, A., Xu, W., Lane, D. P., Spurr, N. K. Chromosome mapping of the human gene encoding the 68-kDa nuclear antigen (p68) by using the polymerase chain reaction. Proc. Nat. Acad. Sci. 86: 6211-6214, 1989.

Gene Name :

DDX5

Protein Name :

Probable ATP-dependent RNA helicase DDX5

Gene Full Name :

DEAD(Asp-Glu-Ala-Asp) box helicase 5

Synonyms :

ATP dependent RNA helicase DDX5 antibody DDX 5 antibody Ddx5 antibody DDX5_HUMAN antibody DEAD(Asp Glu Ala Asp) box helicase 5 antibody DEAD(Asp Glu Ala Asp) box polypeptide 5 antibody DEAD box 5 antibody DEAD box protein 5 antibody DEAD/H(Asp Glu Ala Asp/His) box polypeptide 5(RNA helicase, 68kD) antibody DEAD/H(Asp Glu Ala Asp/His) box polypeptide 5 antibody DEAD/H(Asp-Glu-Ala-Asp/His) box polypeptide 5(RNA helicase 68kDa) antibody DEAD/H box-5(RNA helicase 68kD) antibody DKFZp686J01190 antibody G17P1 antibody HELR antibody HLR 1 antibody HLR1 antibody HUMP68 antibody P68 antibody p68 RNA helicase antibody Probable ATP dependent RNA helicase DDX5 antibody Probable ATP-dependent RNA helicase DDX5 antibody RNA helicase 68kD antibody RNA helicase p68 antibody RNA-dependent ATPase antibody

Uniprot ID :

P17844

more info or order :

Boster Immunoleader product webpage