Boster Immunoleader

antibody

Anti-Caspase-3(P17) Antibody

PA1961-1

100 ug/vial

200 USD

polyclonal

rabbit

mouse, rat

western blot, immunohistochemistry - paraffin section

Anti-Caspase-3(P17) Antibody

PA1961-1

Polyclonal

Rabbit IgG polyclonal antibody for Caspase-3(CASP3) detection. Tested with WB, IHC-P in Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: rat Predicted to work with: mouse

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Mouse, Rat

Concentration: 0.5-1μg/ml; Tested Species: Rat; Predicted Species: Mouse

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the N-terminus of mouse Caspase-3(P17)(67-81aa TDVDAANLRETFMGL), different from the related rat sequence by one amino acid.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

CASP3(Caspase3 Apoptosis-Related Cysteine Protease), also known as YAMA, CPP32 or APOPAIN, is a caspase protein that interacts with caspase 8 and caspase 9. It is encoded by the CASP3 gene. The CASP3 protein is a member of the cysteine-aspartic acid protease(caspase) family. Tiso et al.(1996) used radiation hybrid mapping to localize the CPP32 gene to human chromosome 4q33-q35.1. Fernandes-Alnemri et al.(1994) found that overexpression of CPP32 in insect cells induced apoptosis. Coexpression of the 2 CPP32 subunits in insect cells also resulted in apoptosis. Tewari et al.(1995) showed that purified human ICE cleaved the Yama proenzyme into a proteolytically active form and that activated Yama cleaved PARP into the 85-kD apoptotic form.

1. Fernandes-Alnemri, T., Litwack, G., Alnemri, E. S. CPP32, a novel human apoptotic protein with homology to Caenorhabditis elegans cell death protein Ced-3 and mammalian interleukin-1 beta-converting enzyme. J. Biol. Chem. 269: 30761-30764, 1994. 2. Tewari, M., Quan, L. T., O'Rourke, K., Desnoyers, S., Zeng, Z., Beidler, D. R., Poirier, G. G., Salvesen, G. S., Dixit, V. M. Yama/CPP32-beta, a mammalian homolog of CED-3, is a CrmA-inhibitable protease that cleaves the death substrate poly(ADP-ribose) polymerase. Cell 81: 801-809, 1995. 3. Tiso, N., Pallavicini, A., Muraro, T., Zimbello, R., Apolloni, E., Valle, G., Lanfranchi, G., Danieli, G. A. Chromosomal localization of the human genes, CPP32, Mch2, Mch3, and Ich-1, involved in cellular apoptosis. Biochem. Biophys. Res. Commun. 225: 983-989, 1996.

CASP3

Caspase-3

caspase 3, apoptosis-related cysteine peptidase

A830040C14Rik antibody Apopain antibody Apopain precursor antibody CASP 3 antibody CASP-3 antibody CASP3 antibody Casp3a antibody Caspase 3 antibody Caspase 3 apoptosis related cysteine protease antibody Caspase 3 p17 subunit antibody Caspase 3, apoptosis-related cysteine peptidase antibody Caspase 3, apoptosis-related cysteine protease antibody Caspase 3, apoptosis-related cysteine protease a antibody Caspase-3 subunit p17 antibody Caspase3 antibody CC3 antibody CPP 32 antibody CPP-32 antibody CPP32 antibody CPP32B antibody Cysteine protease CPP32 antibody EC 3.4.22.56 antibody Human cysteine protease CPP32 isoform alpha mRNA complete cds antibody ICE3 antibody LICE antibody mldy antibody OTTHUMP00000165052 antibody OTTHUMP00000165053 antibody OTTHUMP00000165054 antibody PARP cleavage protease antibody Procaspase3 antibody Protein Yama antibody SCA 1 antibody SCA-1 antibody SCA1 antibody SREBP cleavage activity 1 antibody Yama antibody Yama protein antibody

P70677