Boster Immunoleader

antibody

Anti-MADH7 Antibody

PA1954

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse, rat

western blot, immunohistochemistry - paraffin section

Anti-MADH7 Antibody

PA1954

Polyclonal

Rabbit IgG polyclonal antibody for Mothers against decapentaplegic homolog 7(SMAD7) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, rat Predicted to work with: mouse

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Concentration: 0.5-1μg/ml; Tested Species: Rat; Predicted Species: Human, Mouse

WB: The detection limit for SMAD7 is approximately 2.5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the C-terminus of human MADH7(375-389aa YSLQRPNDHEFMQQP), identical to the related rat and mouse sequences.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

SMAD7(Mother against decapentaplegic drosophila homolog of 7), also known as MADH7 or SMA- AND MAD-RELATED PROTEIN 7, is a protein that in humans is encoded by the SMAD7 gene. It belongs to the SMAD family of proteins, which belong to the TGFbeta superfamily of ligands. By somatic cell hybrid analysis, Topper et al.(1997) mapped the MADH7 gene to human chromosome 18. Topper et al.(1997) demonstrated that MADH7 and MADH6 could form complexes in endothelial cells. MADH7 was induced in cultured vascular endothelium by fluid mechanical forces and was capable of modulating endothelial gene expression in response to both humoral and biomechanical stimuli in vitro. By FISH, Roijer et al.(1998) refined the localization to 18q21.1. Lallemand et al.(2001) showed that cells stably expressing SMAD7 had increased susceptibility to apoptosis induced by TGFB, TNFA, serum withdrawal, or loss of cell adhesion.

1. Lallemand, F., Mazars, A., Prunier, C., Bertrand, F., Kornprost, M., Gallea, S., Roman-Roman, S., Cherqui, G., Atfi, A. Smad7 inhibits the survival nuclear factor kappa-B and potentiates apoptosis in epithelial cells. Oncogene 20: 879-884, 2001. 2. Roijer, E., Moren, A., ten Dijke, P., Stenman, G. Assignment of the Smad7 gene (MADH7) to human chromosome 18q21.1 by fluorescence in situ hybridization. Cytogenet. Cell Genet. 81: 189-190, 1998. 3. Topper, J. N., Cai, J., Qiu, Y., Anderson, K. R., Xu, Y.-Y., Deeds, J. D., Feeley, R., Gimeno, C. J., Woolf, E. A., Tayber, O., Mays, G. G., Sampson, B. A., Schoen, F. J., Gimbrone, M. A., Jr., Falb, D. Vascular MADs: two novel MAD-related genes selectively inducible by flow in human vascular endothelium. Proc. Nat. Acad. Sci. 94: 9314-9319, 1997.

SMAD7

Mothers against decapentaplegic homolog 7

SMAD family member 7

FLJ16482 antibody hSMAD 7 antibody hSMAD7 antibody MAD(mothers against decapentaplegic Drosophila) homolog 7 antibody MAD antibody Mad homolog 7 antibody MAD homolog 8 antibody MAD mothers against decapentaplegic homolog 7 antibody MADH 7 antibody MADH 8 antibody MADH6 antibody MADH8 antibody Mothers Against Decapentaplegic Drosophila Homolog of 6 antibody Mothers Against Decapentaplegic Drosophila Homolog of 7 antibody Mothers against decapentaplegic homolog 7 antibody Mothers against decapentaplegic homolog 8 antibody Mothers against DPP homolog 7 antibody Mothers against DPP homolog 8 antibody SMA- AND MAD-RELATED PROTEIN 7 antibody SMAD 7 antibody SMAD antibody SMAD family member 7 antibody SMAD, mothers against DPP homolog 7(Drosophila) antibody SMAD, mothers against DPP homolog 7 antibody SMAD6 antibody SMAD7 antibody SMAD7_HUMAN antibody

O15105