Anti-OSBP1 Antibody | Boster Immunoleader PA1949 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-OSBP1 Antibody

catalog :

PA1949

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-OSBP1 Antibody

Catalog Number :

PA1949

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Oxysterol-binding protein 1(OSBP) detection. Tested with WB in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, rat Predicted to work with: mouse

Applications :

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence in the middle region of human OSBP1(290-304aa WQKSLQYERDQRIRL), identical to the related rat and mouse sequences.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Background :

OSBP(Oxysterol-Binding Protein), also called OSBP1, is a protein that in humans is encoded by the OSBP gene. Im et al.(2005) reported the structure of the full-length yeast oxysterol-binding-protein Osh4, a member of the OSBP-related protein(ORP) family, at 1.5- to 1.9-angstrom resolution in complexes with ergosterol, cholesterol, and 7-, 20- and 25-hydroxy cholesterol. Moreira et al.(2001) refined the localization of the OSBP1 gene to chromosome 11q12.1 by radiation hybrid analysis. Wang et al.(2005) found that OSBP functions as a cholesterol-binding scaffolding protein coordinating the activity of 2 phosphatases to control the extracellular signal-regulated kinase(ERK) signaling pathway. Cytosolic OSBP formed an approximately 440-kD oligomer with a member of the PTPPBS family of tyrosine phosphatases, the serine/threonine phosphatase PP2A, and cholesterol.

Reference :

1. Im, Y. J., Raychaudhuri, S., Prinz, W. A., Hurley, J. H. Structural mechanism for sterol sensing and transport by OSBP-related proteins. Nature 437: 154-158, 2005. 2. Moreira, E. F., Jaworski, C., Li, A., Rodriguez, I. R. Molecular and biochemical characterization of a novel oxysterol-binding protein (OSBP2) highly expressed in retina. J. Biol. Chem. 276: 18570-18578, 2001. 3. Wang, P., Weng, J., Anderson, R. G. W. OSBP is a cholesterol-regulated scaffolding protein in control of ERK1/2 activation. Science 307: 1472-1476, 2005.

Gene Name :

OSBP

Protein Name :

Oxysterol-binding protein 1

Gene Full Name :

oxysterol binding protein

Synonyms :

OSBP 1 antibody OSBP antibody OSBP1_HUMAN antibody Oxysterol binding protein 1 antibody Oxysterol-binding protein 1 antibody

Uniprot ID :

P22059

more info or order :

Boster Immunoleader product webpage