Boster Immunoleader

antibody

Anti-NFkB p100/p52 Antibody

PA1946

100 ug/vial

200 USD

polyclonal

rabbit

mouse, rat

western blot, immunohistochemistry - paraffin section

Anti-NFkB p100/p52 Antibody

PA1946

Polyclonal

Rabbit IgG polyclonal antibody for Nuclear factor NF-kappa-B p100 subunit(NFKB2) detection. Tested with WB, IHC-P in Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: mouse, rat

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Mouse; Predicted Species: Rat

Concentration: 0.5-1μg/ml; Tested Species: Rat; Predicted Species: Mouse

WB: The detection limit for NFKB2 is approximately 0.5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the N-terminus of mouse NFkB p100(5-21aa YDPGLDGIPEYDDFEFS), identical to the related rat sequence.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

NFKB2(Nuclear Factor Kappa-B, Subunit 2), also known as NFKB or p52/p100 SUBUNIT, is a protein that in humans is encoded by the NFKB2 gene. Liptay et al.(1992) mapped the gene for what they called the p49/p100 subunit of NFKB(NFKB2) to chromosome 10 by Southern blot analysis of panels of human/Chinese hamster cell hybrids. By fluorescence in situ hybridization(FISH), they confirmed the localization and mapped the gene with greater resolution to 10q24. NFKB2 appears to be the same as LYT10. Claudio et al.(2002) showed that bone marrow(BM) cells from Nfkb2-deficient mice, but not Nfkb1-deficient mice, failed to increase relative and total IgD-positive transitional-1(T1) stage B cells in response to Baff. In vivo, however, Nfkb2-deficient mice did generate mature B cells, but at reduced numbers. Mice of the aly/aly strain, which are naturally deficient in Nik, and mice of the A/WySNJ strain, which have a mutation in Baffr, also failed to produce T1 B cells in response to Baff. Baff stimulation enhanced expression of Bcl2 in T1 B cells, thereby promoting B-cell survival, and caused the processing of the p100 form of Nfkb2 to p52, which again required Baffr and Nik, but not Nemo(IKKG). Immunoblot analysis showed that BM cells contained primarily p100.

1. Claudio, E., Brown, K., Park, S., Wang, H., Siebenlist, U. BAFF-induced NEMO-independent processing of NF-kappa-B2 in maturing B cells. Nature Immun. 3: 958-965, 2002. 2. Liptay, S., Schmid, R. M., Perkins, N. D., Meltzer, P., Altherr, M. R., McPherson, J. D., Wasmuth, J. J., Nabel, G. J. Related subunits of NF-kappa-B map to two distinct loci associated with translocations in leukemia, NFKB1 and NFKB2. Genomics 13: 287-292, 1992. 3. Tucker, E., O'Donnell, K., Fuchsberger, M., Hilton, A. A., Metcalf, D., Greig, K., Sims, N. A., Quinn, J. M., Alexander, W. S., Hilton, D. J., Kile, B. T., Tarlinton, D. M., Starr, R. A novel mutation in the Nfkb2 gene generates an NF-kappa-B2 'super repressor' J. Immun. 179: 7514-7522, 2007.

NFKB2

Nuclear factor NF-kappa-B p100 subunit

nuclear factor of kappa light polypeptide gene enhancer in B-cells 2(p49/p100)

DNA binding factor KBF2 antibody DNA-binding factor KBF2 antibody H2TF1 antibody Lymphocyte translocation chromosome 10 antibody Lymphocyte translocation chromosome 10 protein antibody Lyt 10 antibody Lyt10 antibody NF kB2 antibody NFKB2 antibody NFKB2_HUMAN antibody Nuclear factor NF kappa B p100 subunit antibody Nuclear factor NF kappa B p52 subunit antibody Nuclear factor NF-kappa-B p52 subunit antibody Nuclear factor of kappa light chain gene enhancer in B cells 2 antibody Nuclear factor of kappa light polypeptide gene enhancer in B cells 2 antibody Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 antibody Oncogene Lyt 10 antibody Oncogene Lyt-10 antibody p105 antibody p49/p100 antibody

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