Boster Immunoleader

antibody

Anti-Cullin3 Antibody

PA1939

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse, rat

info changed 20150325

Anti-Cullin3 Antibody

PA1939

Polyclonal

Rabbit IgG polyclonal antibody for Cullin-3(CUL3) detection. Tested with WB in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, rat Predicted to work with: mouse

WB

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

WB: The detection limit for CUL3 is approximately 0.5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence in the middle region of human Cullin 3(426-444aa DVFERYYKQHLARRLLTNK), identical to the related rat and mouse sequences.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Cullin-3(Cul 3), is a protein that in humans is encoded by the CUL3 gene. Kipreos et al.(1996) identified a conserved gene family, designated cullins, with at least 5 members in nematodes, 6 in humans, and 3 in S. cerevisiae. Human CUL3 is an ortholog of nematode cul3. Hartz(2012) mapped the CUL3 gene to chromosome 2q36.2 based on an alignment of the CUL3 sequence with the genomic sequence(GRCh37). Sumara et al.(2007) found that KLHL9, KLHL13, and CUL3 interacted directly in a 370-kD protein complex in HeLa cell lysates. Rondou et al.(2008) showed that interaction between KLHL12 and the CUL3 ubiquitin ligase complex directed ubiquitination of dopamine receptor D4(DRD4). KLHL12 interacted directly with CUL3 and with the polymorphic intracellular loop-3 of D4. By immunoprecipitation analysis of HeLa cells and HCT116 human colon carcinoma cells expressing epitope-tagged proteins, Kigoshi et al.(2011) showed that KLHL7 interacted with CUL3 and ROC1(RBX1).

1. Kigoshi, Y., Tsuruta, F., Chiba, T. Ubiquitin ligase activity of Cul3-KLHL7 protein is attenuated by autosomal dominant retinitis pigmentosa causative mutation. J. Biol. Chem. 286: 33613-33621, 2011. 2. Kipreos, E. T., Lander, L. E., Wing, J. P., He, W. W., Hedgecock, E. M. cul-1 is required for cell cycle exit in C. elegans and identifies a novel gene family. Cell 85: 829-839, 1996. 3. Rondou, P., Haegeman, G., Vanhoenacker, P., Van Craenenbroeck, K. BTB protein KLHL12 targets the dopamine D4 receptor for ubiquitination by a Cul3-based E3 ligase. J. Biol. Chem. 283: 11083-11096, 2008. 4. Sumara, I., Quadroni, M., Frei, C., Olma, M. H., Sumara, G., Ricci, R., Peter, M. A Cul3-based E3 ligase removes Aurora B from mitotic chromosomes, regulating mitotic progression and completion of cytokinesis in human cells. Dev. Cell 12: 887-900, 2007.

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Q13618