Boster Immunoleader

antibody

Anti-MEKK1 Antibody

PA1923

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse, rat

western blot, immunohistochemistry - paraffin section

Anti-MEKK1 Antibody

PA1923

Polyclonal

Rabbit IgG polyclonal antibody for Mitogen-activated protein kinase kinase kinase 1(MAP3K1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human Predicted to work with: mouse, rat

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Human; Predicted Species: Mouse, Rat

Concentration: 0.5-1μg/ml; Tested Species: Human; Predicted Species: Mouse, Rat

WB: The detection limit for MAP3K1 is approximately 0.5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the C-terminus of human MEKK1(1418-1432aa PEVLRGQQYGRSCDV), identical to the related rat and mouse sequences.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

MAP3K1(Mitogen-activated protein kinase kinase kinase 1), also known as MEKK1, MAPKKK1, MEK KINASE or MAP/ERK KINASE KINASE 1, is an enzyme that in humans is encoded by the MAP3K1 gene. Vinik et al.(1995) identified DNA sequence and size polymorphisms in intronic and 3-prime untranslated regions of the mouse Map3k1 gene and the human MAP3K1 homolog. Using these allele-specific polymorphisms, they mapped the Map3k1 gene in an intersubspecific backcross to mouse chromosome 13. They mapped the human MAP3K1 gene to chromosome 5 by somatic cell hybrid analysis. By assaying transfected COS-1 cells, Xia et al.(1998) showed that human MEKK1 activated JNK1(MAPK8) robustly and p38-alpha(MAPK14) less efficiently, but it had only a marginal effect on ERK2(MAPK1). MEKK1 directly and specifically interacted with JNKK1(MAP2K4) and activated JNKK1 in cells and in vitro. Phosphorylation of JNKK1 by MEKK1 disrupted their interaction. MEKK1 and JNK1 competed for binding to JNKK1. Xia et al.(1998) concluded that JNKK1 is the preferred MEKK1 substrate.

1. Juriloff, D. M., Harris, M. J., Mah, D. G. The open-eyelid mutation, lidgap-Gates, is an eight-exon deletion in the mouse Map3k1 gene. Genomics 85: 139-142, 2005. 2. Vinik, B. S., Kay, E. S., Fiedorek, F. T., Jr. Mapping of the MEK kinase gene (Mekk) to mouse chromosome 13 and human chromosome 5. Mammalian Genome 6: 782-783, 1995. 3. Xia, Y., Wu, Z., Su, B., Murray, B., Karin, M. JNKK1 organizes a MAP kinase module through specific and sequential interactions with upstream and downstream components mediated by its amino-terminal extension. Genes Dev. 12: 3369-3381, 1998.

MAP3K1

Mitogen-activated protein kinase kinase kinase 1

mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase

M3K1_HUMAN antibody MAP3K1 antibody MAPK/ERK kinase kinase 1 antibody MAPKKK1 antibody MEK kinase 1 antibody MEKK 1 antibodyMekk antibody MEKK1 antibody Mitogen activated protein kinase kinase kinase 1 antibody Mitogen activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase antibody Mitogen-activated protein kinase kinase kinase 1 antibodySRXY6 antibody

Q13233