Boster Immunoleader

antibody

Anti-MEK6 Antibody

PA1921

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse, rat

Anti-MEK6 Antibody

PA1921

Polyclonal

Rabbit IgG polyclonal antibody for Dual specificity mitogen-activated protein kinase kinase 6(MAP2K6) detection. Tested with WB in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, mouse, rat

WB

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Mouse, Rat

WB: The detection limit for MAP2K6 is approximately 0.5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the N-terminus of human MEK6(7-23aa KKRNPGLKIPKEAFEQP), identical to the related mouse sequence and different from the related rat sequence by one amino acid.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

MAP2K6(Mitogen-activated protein kinase kinase 6), also known as MAP kinase kinase 6(MAPKK 6) or MAPK/ERK kinase 6 is an enzyme that in humans is encoded by the MAP2K6 gene, on chromosome 17. MAPKK 6 is a member of the dual specificity protein kinase family, which functions as a mitogen-activated protein(MAP) kinase kinase. Han et al.(1996) described experiments addressing the function of MKK6 in intracellular signaling pathways leading toward activation of p38 MAPK. Mukherjee et al.(2006) found that the Yersinia YopJ virulence factor acted as an acetyltransferase, using coenzyme A to modify critical ser and thr residues in the MAP2K6 activation loop, thereby blocking phosphorylation and kinase activation. They proposed that ser and/or thr acetylation by a bacterial effector may be a mechanism that occurs in other signaling pathways.

1. Han, J., Lee, J.-D., Jiang, Y., Li, Z., Feng, L., Ulevitch, R. J. Characterization of the structure and function of a novel MAP kinase kinase (MKK6). J. Biol. Chem. 271: 2886-2891, 1996. 2. Mao, Z., Bonni, A., Xia, F., Nadal-Vicans, M., Greenberg, M. E. Neuronal activity-dependent cell survival mediated by transcription factor MEF2. Science 286: 785-790, 1999. 3. Mukherjee, S., Keitany, G., Li, Y., Wang, Y., Ball, H. L., Goldsmith, E. J., Orth, K. Yersinia YopJ acetylates and inhibits kinase activation by blocking phosphorylation. Science 312: 1211-1214, 2006.

MAP2K6

Dual specificity mitogen-activated protein kinase kinase 6

mitogen-activated protein kinase kinase 6

Dual specificity mitogen activated protein kinase kinase 6 antibody Dual specificity mitogen-activated protein kinase kinase 6 antibody EC 2.7.12.2 antibody MAP kinase kinase 6 antibody MAP2K6 antibody MAPK/ERK kinase 6 antibody MAPKK 6 antibody MAPKK6 antibody MEK 6 antibody MEK6 antibody Mitogen Activated Protein Kinase Kinase 6 antibody MKK 6 antibody MKK6 antibody MP2K6_HUMAN antibody PRKMK6 antibody Protein kinase mitogen activated kinase 6 antibody protein kinase, mitogen-activated, kinase 6(MAP kinase kinase 6) antibody SAPK kinase 3 antibody SAPKK-3 antibody SAPKK3 antibody SKK3 antibody Stress-activated protein kinase kinase 3 antibody

P52564