Anti-Ki67 Antibody | Boster Immunoleader PA1918 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Ki67 Antibody

catalog :

PA1918

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human

application :

western blot

publications citing this reagent :

Bao J, Tu Z, Sun H, Luo G, Yang L, Song J, et al. R2: identification of renal potential progenitor/stem cells that participate in the renal regeneration processes of kidney allograft fibrosis. Nephrology (Carlton). 2008;13:500-7 pubmed publisher

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Ki67 Antibody

Catalog Number :

PA1918

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Antigen KI-67(MKI67) detection. Tested with WB in Human.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human

Applications :

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Notes :

WB: The detection limit for MKI67 is approximately 1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human Ki67(3234-3256aa KKAEDNVCVKKIRTRSHRDSEDI).

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Background :

Ki-67(Proliferation-related Ki-67 antigen), also known as MKI67 or KIA, is a protein that in humans is encoded by the MKI67 gene. From study of a panel of human-rodent somatic cell hybrids, Schonk et al.(1989) demonstrated that a gene involved in the expression of the MKI67 antigen is located on chromosome 10. By in situ hybridization, Fonatsch et al.(1991) regionalized the MKI67 gene to chromosome 10q25-qter. By FISH, Traut et al.(1998) mapped the mouse Mki67 gene to chromosome 7F3-F5. Antigen KI-67 is a nuclear protein that is associated with and may be necessary for cellular proliferation. Furthermore it is associated with ribosomal RNA transcription. Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis.

Reference :

1. Fonatsch, C., Duchrow, M., Rieder, H., Schluter, C., Gerdes, J. Assignment of the human Ki-67 gene (MKI67) to 10q25-qter. Genomics 11: 476-477, 1991. 2. Schonk, D. M., Kuijpers, H. J. H., vanDrunen, E., vanDalen, C. H., Geurts van Kessel, A. H. M., Verheijen, R., Ramaekers, F. C. S. Assignment of the gene(s) involved in the expression of the proliferation-related Ki-67 antigen to human chromosome 10. Hum. Genet. 83: 297-299, 1989. 3. Traut, W., Scholzen, T., Winking, H., Kubbutat, M. H. G., Gerdes, J. Assignment of the murine Ki-67 gene (Mki67) to chromosome band 7F3-F5 by in situ hybridization. Cytogenet. Cell Genet. 83: 12-13, 1998.

Gene Name :

MKI67

Protein Name :

Antigen KI-67

Gene Full Name :

antigen identified by monoclonal antibody Ki-67

Synonyms :

Antigen identified by monoclonal antibody Ki 67 antibody Antigen KI-67 antibody Antigen KI67 antibody KI67_HUMAN antibody KIA antibody MKI67 antibody Proliferation related Ki 67 antigen antibody RP11-380J17.2 antibody

Uniprot ID :

P46013

more info or order :

Boster Immunoleader product webpage