Boster Immunoleader

antibody

Anti-Iduronate 2 sulfatase Antibody

PA1917

100 ug/vial

200 USD

polyclonal

rabbit

western blot, immunohistochemistry - paraffin section

Anti-Iduronate 2 sulfatase Antibody

PA1917

Polyclonal

Rabbit IgG polyclonal antibody for Iduronate 2-sulfatase(IDS) detection. Tested with WB, IHC-P in Human.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Concentration: 0.5-1μg/ml; Tested Species: Human

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the C-terminus of human Iduronate 2 sulfatase(430-448aa ELCREGKNLLKHFRFRDLE).

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

IDS(Iduronate-2-sulfatase) is a sulfatase enzyme associated with Hunter syndrome. Iduronate 2-sulfatase is involved in the lysosomal degradation of the glycosaminoglycans heparan sulfate and dermatan sulfate. Wilson et al.(1991) used an IDS cDNA clone to localize the gene to Xq28, distal to the fragile X site. Faust et al.(1992) and Daniele et al.(1993) demonstrated that the homologous Ids gene in the mouse occupies the same position on the X chromosome in relation to the FMR1, F9, and GABRA3 genes. Iduronate-2-sulfatase is required for the lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations in this X-chromosome gene that result in enzymatic deficiency lead to the sex-linked mucopolysaccharidosis type II, also known as Hunter syndrome. Iduronate-2-sulfatase has a strong sequence homology with human arylsulfatases A, B, and C, and human glucosamine-6-sulfatase.

1. Daniele, A., Faust, C. J., Herman, G. E., Di Natale, P., Ballabio, A. Cloning and characterization of the cDNA for the murine iduronate sulfatase gene. Genomics 16: 755-757, 1993. 2. Faust, C. J., Verkerk, A. J. M. H., Wilson, P. J., Morris, C. P., Hopwood, J. J., Oostra, B. A., Herman, G. E. Genetic mapping on the mouse X chromosome of human cDNA clones for the fragile X and Hunter syndromes. Genomics 12: 814-817, 1992. 3. Wilson, P. J., Suthers, G. K., Callen, D. F., Baker, E., Nelson, P. V., Cooper, A., Wraith, J. E., Sutherland, G. R., Morris, C. P., Hopwood, J. J. Frequent deletions at Xq28 indicate genetic heterogeneity in Hunter syndrome. Hum. Genet. 86: 505-508, 1991.

IDS

Iduronate 2-sulfatase

iduronate 2-sulfatase

Alpha L iduronate sulfate sulfatase antibody Alpha-L-iduronate sulfate sulfatase antibody AW214631 antibody Ids antibody IDS_HUMAN antibody Iduronate 2 sulfatase 14 kDa chain antibody Iduronate 2 sulfatase 42 kDa chain antibody Iduronate 2 sulfatase antibody Iduronate 2-sulfatase 14 kDa chain antibody Iduronate sulfatase antibody Idursulfase antibody MPS2 antibody RP23-29M4.1 antibody SIDS antibody

P22304