Anti-Fibroblast activation protein, alpha Antibody | Boster Immunoleader PA1913 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Fibroblast activation protein, alpha Antibody

catalog :

PA1913

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

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product information

Product Name :

Anti-Fibroblast activation protein, alpha Antibody

Catalog Number :

PA1913

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Seprase(FAP) detection. Tested with WB, IHC-P, IHC-F in Human;Mouse;rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, mouse, rat

Applications :

WB,IHC-P,IHC-F

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Mouse; Predicted Species: Rat

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human, Mouse, Rat

Immunohistochemistry(Frozen Section) :

Concentration: 0.5-1μg/ml; Tested Species: Rat; Predicted Species: Human, Mouse

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the N-terminus of human Fibroblast activation protein, alpha(57-73aa FFPNWISGQEYLHQSAD), different from the related rat and mouse sequences by three amino acids.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P) and IHC(F).

Background :

FAP(Fibroblast Activation Protein, Alpha) also known as FAPA or SEPRASE, is an inducible cell surface glycoprotein that was originally identified in cultured fibroblasts using monoclonal antibody F19. The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. The FAP gene is mapped on 2q24.2. FAP is most closely related to DPPIV and they share about 50% of their amino acids. FAP is catalytically active as a 170kD dimer and has dipeptidase and gelatinase activity. Its gelatinase activity requires a glycine in P2 position.FAP-alpha shows 48% amino acid identity with dipeptidyl peptidase IV and 30% identity with DPP4-related protein. Northern blot analysis detected a 2.8-kb FAP-alpha mRNA in fibroblasts. Depletion of FAP-expressing cells, which made up only 2% of all tumor cells in established Lewis lung carcinomas, caused rapid hypoxic necrosis of both cancer and stromal cells in immunogenic tumors by a process involving interferon-gamma and tumor necrosis factor-alpha.

Reference :

1. Goldstein, L. A., Ghersi, G., Pineiro-Sanchez, M. L., Salamone, M., Yeh, Y., Flessate, D., Chen, W.-T. Molecular cloning of seprase: a serine integral membrane protease from human melanoma.Biochim. Biophys. Acta 1361: 11-19, 1997. 2. Mathew, S., Scanlan, M. J., Mohan Raj, B. K., Murty, V. V. V. S., Garin-Chesa, P., Old, L. J., Rettig, W. J., Chaganti, R. S. K. The gene for fibroblast activation protein alpha (FAP), a putative cell surface-bound serine protease expressed in cancer stroma and wound healing, maps to chromosome band 2q23. Genomics 25: 335-337, 1995. 3. Scanlan, M. J., Raj, B. K. M., Calvo, B., Garin-Chesa, P., Sanz-Moncasi, M. P., Healey, J. H., Old, L. J., Rettig, W. J. Molecular cloning of fibroblast activation protein alpha, a member of the serine protease family selectively expressed in stromal fibroblasts of epithelial cancers. Proc. Nat. Acad. Sci. 91: 5657-5661, 1994.

Gene Name :

FAP

Protein Name :

Seprase

Gene Full Name :

fibroblast activation protein, alpha

Synonyms :

170 kDa melanoma membrane bound gelatinase antibody 170 kDa melanoma membrane-bound gelatinase antibody DPPIV antibody FAP antibody FAPA antibody Fibroblast activation protein alpha antibody Integral membrane serine protease antibody SEPR_HUMAN antibody Seprase antibody

Uniprot ID :

Q12884

more info or order :

Boster Immunoleader product webpage