Boster Immunoleader

antibody

Anti-DLL3 Antibody

PA1912

100 ug/vial

200 USD

polyclonal

rabbit

Anti-DLL3 Antibody

PA1912

Polyclonal

Rabbit IgG polyclonal antibody for Delta-like protein 3(DLL3) detection. Tested with WB in Human.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human

WB

Concentration: 0.1-0.5μg/ml; Tested Species: Human

WB: The detection limit for DLL3 is approximately 0.5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the C-terminus of human DLL3(599-618aa RAGQRQHLLFPYPSSILSVK).

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

DLL3(DELTA-LIKE 3) also known as DELTA, DROSOPHILA, HOMOLOG OF, is a protein which in humans is encoded by the DLL3 gene. This gene encodes a member of the delta protein ligand family. This family functions as Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain. Mutation in the mouse delta-like 3 gene(Dll3), which is homologous to the Notch-ligand delta in Drosophila, results in the mouse 'pudgy' phenotype. The human DLL3 gene was identified within a critical interval, mapped in 2 consanguineous Arab-Israeli and Pakistani SCDO1 pedigrees, of 7.8 cM at 19q13.1-q13.3 between D19S570 and D19S908(Bulman et al., 2000). Dunwoodie et al.(1997) presented results suggesting that mouse Dll3 may complement the function of other delta homologs during early pattern formation in the mouse embryo. In humans, the fact that mutations in genes required for oscillation, such as DLL3, result in abnormal segmentation of the vertebral column suggests that the segmentation clock also acts during human embryonic development. This residue is highly conserved in Delta proteins from Drosophila to humans, and the substitution of a charged polar for a nonpolar residue may disrupt the conformation of the DLL3 protein.

1. Bulman, M. P., Kusumi, K., Frayling, T. M., McKeown, C., Garrett, C., Lander, E. S., Krumlauf, R., Hattersley, A. T., Ellard, S., Turnpenny, P. D. Mutations in the human delta homologue,DLL3, cause axial skeletal defects in spondylocostal dysostosis.Nature Genet. 24: 438-441, 2000. 2. Dunwoodie, S. L., Henrique, D., Harrison, S. M., Beddington, R. S. P. Mouse Dll3: a novel divergent delta gene which may complement the function of other delta homologues during early pattern formation in the mouse embryo. Development 124: 3065-3076, 1997. 3. Kusumi, K., Sun, E. S., Kerrebrock, A. W., Bronson, R. T., Chi, D.-C., Bulotsky, M. S., Spencer, J. B., Birren, B. W., Frankel, W. N., Lander, E. S. The mouse pudgy mutation disrupts Delta homologue Dll3 and initiation of early somite boundaries.Nature Genet. 19: 274-278, 1998.

DLL3

Delta-like protein 3

delta-like 3(Drosophila)

Delta Drosophila like 3 antibody Delta like 3 Drosophila antibody Delta like 3 homolog Drosophila antibody Delta like 3 protein antibody Delta like protein 3 precursor antibody Delta-like protein 3 antibody Delta3 antibody Dll3 antibody DLL3_HUMAN antibody Drosophila Delta homolog 3 antibody SCDO1 antibody SCOD1 antibody Spondylocostal dysostosis autosomal recessive antibody

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