Boster Immunoleader

antibody

Anti-DCC Antibody

PA1910

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse, rat

western blot, immunohistochemistry - paraffin section

Anti-DCC Antibody

PA1910

Polyclonal

Rabbit IgG polyclonal antibody for Netrin receptor DCC(DCC) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, mouse, rat

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Mouse, Rat

Concentration: 0.5-1μg/ml; Tested Species: Human; Predicted Species: Mouse, Rat

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the N-terminus of human DCC(162-178aa EVIGEPMPTIHWQKNQQ), identical to the related mouse sequence, and different from the related rat sequence by one amino acid.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

DCC(Deleted IN Colorectal Carcinoma) also known as CRC18 or CRCR1. The DCC gene encodes a functional receptor for netrin and mediates axon outgrowth and the steering response. Heterozygous loss-of-function mutations in DCC can result in congenital mirror movements. Alterations in DCC occur frequently in colorectal cancer. Studying a YAC contig containing the entire DCC coding region, they showed that the DCC gene spans approximately 1.4 Mb. Vogelstein(1995) stated that the precise location of the DCC gene was though to be 18q21.3. DCC is a receptor or a component of a receptor that mediates the effects of netrin-1 on commissural axons, and they complement genetic evidence for interactions between DCC and netrin homologs in C. DCC protein could be detected in varying abundance in all specimens of normal colonic mucosa analyzed as well as in all specimens of adenomatous polyps, colorectal carcinoma and colorectal liver metastases. DCC may function as a tumor-suppressor protein by inducing apoptosis in settings in which ligand is unavailable(for example, during metastasis or tumor growth beyond local blood supply) through functional caspase cascades by a mechanism that requires cleavage of DCC at asp1290. Stein et al.(2001) concluded that DCC plays a central role in netrin signaling of axon growth and guidance independent of A2B receptor activation.

1. Castets, M., Broutier, L., Molin, Y., Brevet, M., Chazot, G., Gadot, N., Paquet, A., Mazelin, L., Jarroson-Wuilleme, L., Scoazec, J.-Y., Bernet, A., Mehlen, P. DCC constrains tumour progression via its dependence receptor activity. Nature 482: 534-537, 2012. 2. Stein, E., Tessier-Lavigne, M. Hierarchical organization of guidance receptors: silencing of netrin attraction by Slit through a Robo/DCC receptor complex. Science 291: 1928-1938, 2001. 3. Vogelstein, B. Personal Communication. Baltimore, Md. 11/30/1995.

DCC

Netrin receptor DCC

deleted in colorectal carcinoma

Colorectal cancer related chromosome sequence 18 antibody Colorectal cancer suppressor antibody CRC 18 antibody CRC18 antibody CRCR 1 antibody CRCR1 antibody DCC antibody DCC_HUMAN antibody Deleted in colorectal cancer protein antibody Deleted in colorectal carcinoma antibody Immunoglobulin superfamily DCC subclass member 1 antibody Netrin receptor DCC antibody Tumor suppressor protein DCC antibody

P43146