Boster Immunoleader

antibody

Anti-Cytochrome P450 2E1 Antibody

PA1909

100 ug/vial

200 USD

polyclonal

rabbit

mouse, rat

western blot, immunohistochemistry - paraffin section

Anti-Cytochrome P450 2E1 Antibody

PA1909

Polyclonal

Rabbit IgG polyclonal antibody for Cytochrome P450 2E1(CYP2E1) detection. Tested with WB, IHC-P in Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: mouse, rat

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Mouse, Rat

Concentration: 0.5-1μg/ml; Tested Species: Mouse, Rat

WB: The detection limit for CYP2E1 is approximately 1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the N-terminus of mouse Cytochrome P450 2E1(94-112aa KNEFSGRGDIPVFQEYKNK), identical to the related rat sequence.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

CYP2E1, also known as P450IIE1, is a member of the P450IIE subfamily which is ethanol-inducible. It has at least 1 gene which is mapped to 10q24.3-qter, and a second is likely in rat and in man. Both the rat and human proteins encoded by this gene contain 493 amino acids and calculated molecular masses of 56,634 and 56,916 daltons, respectively. In addition, genetic polymorphisms in the 5-prime flanking region of the human P450IIE1 gene affected its binding of transacting factor and changed its transcriptional regulation, which may lead to interindividual differences of microsomal drug oxidation activity. P450IIE1 is an important enzyme for the catalysis of the conversion of ethanol to acetaldehyde and to acetate in humans,and it is also involved in the metabolism of nitrosamines. Due to the possible correlation of P450IIE1 genes with malignancy, clinical studies of RFLP patterns of these genes in cancer may be useful.

1. Nebert, D. W.; Adesnik, M.; Coon, M. J.; Estabrook, R. W.; Gonzalez, F. J.; Guengerich, F. P.; Gunsalus, I. C.; Johnson, E. F.; Kemper, B.; Levin, W.; Phillips, I. R.; Sato, R.; Waterman, M. R. : The P450 gene superfamily: recommended nomenclature. DNA 6: 1-11, 1987. 2. Song, B.-J.; Gelboin, H. V.; Park, S.-S.; Yang, C. S.; Gonzalez, F. J. : Complementary DNA and protein sequences of ethanol-inducible rat and human cytochrome P-450s: transcriptional and post-transcriptional regulation of the rat enzyme. J. Biol. Chem. 261: 16689-16697, 1987. 3. Hayashi, S.; Watanabe, J.; Kawajiri, K. : Genetic polymorphisms in the 5-prime-flanking region change transcriptional regulation of the human cytochrome P450IIE1 gene. J. Biochem. 110: 559-565, 1991.

CYP2E1

Cytochrome P450 2E1

cytochrome P450, family 2, subfamily E, polypeptide 1

4-nitrophenol 2-hydroxylase antibody CP2E1_HUMAN antibody CPE 1 antibody CPE1 antibody CYP2E antibody CYP2E1 antibody CYPIIE1 antibody Cytochrome P450 2E1 antibody Cytochrome P450 family 2 subfamily E polypeptide 1 antibody Cytochrome P450 isozyme 3A antibody Cytochrome P450 LM3A antibody Cytochrome P450 subfamily IIE(ethanol inducible) polypeptide 1 antibody Cytochrome P450 subfamily IIE polypeptide 1 antibody Cytochrome P450, family 2, subfamily e, polypeptide 1 antibody cytochrome P450, subfamily IIE(ethanol-inducible), polypeptide 1 antibody Cytochrome P450, subfamily IIE antibody Cytochrome P450-ALC antibody Cytochrome P450-J antibody Cytochrome P450RLM6 antibody EC 1.14.13.- antibody EC 1.14.13.n7 antibody Ethanol-inducible P450 antibody Flavoprotein linked monooxygenase antibody Flavoprotein-linked monooxygenase antibody MGC133529 antibody Microsomal monooxygenase antibody OTTHUMP00000020813 antibody OTTHUMP00000046571 antibody P450 ALC antibody p450 antibody P450 J antibody P450-J antibody P450C2E antibody P450J antibody P450RLM6 antibody Xenobiotic monooxygenase antibody

Q05421