Boster Immunoleader

antibody

Anti-Smad2 Antibody

PA1898

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse, rat

western blot, immunocytochemistry, immunohistochemistry - paraffin section

info changed 20150325

Anti-Smad2 Antibody

PA1898

Polyclonal

Rabbit IgG polyclonal antibody for Mothers against decapentaplegic homolog 2(SMAD2) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, rat Predicted to work with: mouse

WB,IHC-P,ICC

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Concentration: 0.5-1μg/ml; Tested Species: Human; Predicted Species: Mouse, Rat

Concentration: 0.5-1μg/ml; Tested Species: Human; Predicted Species: Mouse, Rat

WB: The detection limit for SMAD2 is approximately 2.5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the N-terminus of human Smad2(94-112aa DQWDTTGLYSFSEQTRSLD), identical to the related rat and mouse sequences.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P) and ICC.

Smad2(Mothers against decapentaplegic homolog 2), also known as MADR2, MADH2, SMAD family member 2 or SMAD2, is a protein that in humans is encoded by the SMAD2 gene. MAD homolog 2 belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic'(Mad) and the C. elegans gene Sma. Eppert et al.(1996) mapped the MADR2 gene close to DPC4 at 18q21, a region which is frequently deleted in colorectal cancers. Riggins et al.(1996) mapped the human MADH2 gene to 18q21. Nakao et al.(1997) refined the localization of the SMAD2 gene to 18q21.1, approximately 3 Mb proximal to DPC4, by fluorescence in situ hybridization. SMAD2 mediates the signal of the transforming growth factor(TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation(SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors.

1. Eppert, K., Scherer, S. W., Ozcelik, H., Pirone, R., Hoodless, P., Kim, H., Tsui, L.-C., Bapat, B., Gallinger, S., Andrulis, I. L., Thomsen, G. H., Wrana, J. L., Attisano, L.MADR2 maps to 18q21 and encodes a TGF-beta-regulated MAD-related protein that is functionally mutated in colorectal carcinoma.Cell 86: 543-552, 1996. 2. Nakao, A., Roijer, E., Imamura, T., Souchelnytskyi, S., Stenman, G., Heldin, C.-H., ten Dijke, P.Identification of Smad2, a human Mad-related protein in the transforming growth factor-beta signaling pathway.J. Biol. Chem. 272: 2896-2900, 1997. 3. Riggins, G. J., Thiagalingam, S., Rozenblum, E., Weinstein, C. L., Kern, S. E., Hamilton, S. R., Willson, J. K. V., Markowitz, S. D., Kinzler, K. W., Vogelstein, B.Mad-related genes in the human.Nature Genet. 13: 347-349, 1996.

SMAD2

Mothers against decapentaplegic homolog 2

SMAD family member 2

hMAD 2 antibody hMAD-2 antibody hSMAD2 antibody JV18 1 antibody JV18 antibody JV18-1 antibody JV181 antibody MAD antibody MAD homolog 2 antibody MAD Related Protein 2 antibody Mad-related protein 2 antibody MADH2 antibody MADR2 antibody MGC22139 antibody MGC34440 antibody Mothers Against Decapentaplegic Homolog 2 antibody mothers against DPP homolog 2 antibody OTTHUMP00000163489 antibody Sma and Mad related protein 2 antibody SMAD 2 antibody SMAD antibody SMAD family member 2 antibody SMAD, mothers against DPP homolog 2 antibody SMAD2 antibody SMAD2_HUMAN antibody

Q15796