Anti-SHC Antibody | Boster Immunoleader PA1897 product information

product summary

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company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-SHC Antibody

catalog :

PA1897

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

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product information

Availability :

info changed 20150325

Product Name :

Anti-SHC Antibody

Catalog Number :

PA1897

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for SHC-transforming protein 1(SHC1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, rat Predicted to work with: mouse

Applications :

WB,IHC-P,IHC-F,ICC

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Immunohistochemistry(Frozen Section) :

Concentration: 0.5-1μg/ml; Tested Species: Rat; Predicted Species: Human, Mouse

Immunocytochemistry :

Concentration: 0.5-1μg/ml; Tested Species: Human

Notes :

WB: The detection limit for SHC1 is approximately 1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human SHC(424-440aa DPSYVNVQNLDKARQAV), different from the related rat and mouse sequences by two amino acids.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P), IHC(F) and ICC.

Background :

SHC1(SHC-transforming protein 1), also known as SHCA, is a protein that in humans is encoded by the SHC1 gene. SCOP classifies the 3D structure as belonging to the SH2 domain family. By Southern analysis of somatic cell hybrids followed by both isotopic and fluorescence in situ hybridization, Huebner et al.(1994) assigned the SHC1 gene to 1q21. Yulug et al.(1995) used fluorescence in situ hybridization to map the SHC1 gene to 1q21. By the same method, an SHC-related sequence(SHCL1) was mapped to 17q21-q22. By FISH analysis and direct sequencing of vectorette library PCR products, Harun et al.(1997) identified SHC1P1, a 3.2-kb processed pseudogene, in Xq12-q13.1. SHC1P1 is 85% identical to mouse Shc p66. Reporter assays showed FKHRL1 transactivates CAT, suggesting a capacity to augment antioxidant scavenging. Nemoto and Finkel(2002) concluded that there is an important functional relationship between forkhead proteins(e.g., FKHRL1), SHC1, and intracellular oxidants, all of which are though to be involved in the aging process in worms and mammals.

Reference :

1. Harun, R. B., Smith, K. K., Leek, J. P., Markham, A. F., Norris, A., Morrison, J. F. J.Characterization of human SHC p66 cDNA and its processed pseudogene mapping to Xq12-q13.1.Genomics 42: 349-352, 1997. 2. Huebner, K., Kastury, K., Druck, T., Salcini, A. E., Lanfrancone, L., Pelicci, G., Lowenstein, E., Li, W., Park, S.-H., Cannizzaro, L., Pelicci, P. G., Schlessinger, J.Chromosome locations of genes encoding human signal transduction adapter proteins, Nck (NCK), Shc (SHC1), and Grb2 (GRB2).Genomics 22: 281-287, 1994. 3. Pelicci, G., Lanfrancone, L., Grignani, F., McGlade, J., Cavallo, F., Forni, G., Nicoletti, I., Grignani, F., Pawson, T., Pelicci, P. G.A novel transforming protein (SHC) with an SH2 domain is implicated in mitogenic signal transduction.Cell 70: 93-104, 1992.

Gene Name :

SHC1

Protein Name :

SHC-transforming protein 1

Gene Full Name :

SHC(Src homology 2 domain containing) transforming protein 1

Synonyms :

FLJ26504 antibody MGC98812 antibody p46 antibody p52 antibody p52 SHC antibody p52SHC antibody p66 antibody p66 SHC antibody p66SHC antibody SH2 domain protein C1 antibody SHC(Src homology 2 domain containing) transforming protein 1 antibody SHC 1 antibody SHC A antibody Shc antibody SHC transforming protein 1 antibody SHC transforming protein antibody SHC-transforming protein 1 antibody SHC-transforming protein 3 antibody SHC-transforming protein A antibody SHC1 antibody SHC1_HUMAN antibody SHCA antibody Src homology 2 domain containing transforming protein C1 antibody Src homology 2 domain-containing-transforming protein C1 antibody

Uniprot ID :

P29353

more info or order :

Boster Immunoleader product webpage