Boster Immunoleader

antibody

Anti-Rb2 p130 Antibody

PA1895

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse, rat

Anti-Rb2 p130 Antibody

PA1895

Polyclonal

Rabbit IgG polyclonal antibody for Retinoblastoma-like protein 2(RBL2) detection. Tested with WB in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, mouse, rat

WB

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Mouse, Rat

WB: The detection limit for RBL2 is approximately 1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the C-terminus of human Rb2 p130(1120-1139aa ENHSALLRRLQDVANDRGSH), identical to the related rat sequence and different from the related mouse sequence by one amino acid.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

RBL2(Retinoblastoma-like protein 2) is a protein that in humans is encoded by the RBL2 gene. It is also known as Retionblastoma-related gene(RB2), P130. Yeung et al.(1993) mapped the RBL2 gene to human chromosome 16q12.2 and rat chromosome 19, using fluorescence in situ hybridization and somatic hybrid cell analysis, respectively. Based on known syntenic relationships among human, rat and mouse, the data suggested that the mouse homolog resides on chromosome 8. Deletions of chromosome 16q have been found in several human neoplasms, including breast, ovarian, hepatic, and prostate cancers, which supports the involvement of RB2 in human cancer as a tumor suppressor gene. Kong et al.(2006)found that RBL2 and RINT1 were essential for telomere length control in human fibroblasts, with loss of either protein leading to longer telomeres. Williams et al.(2006)found that mouse fibroblasts lacking Rb were less susceptible to an oncogenic HRAS allele than wildtype cells. In contrast, p107(RBL1;) -/- and p130 -/- fibroblasts were more susceptible to HRAS-mediated transformation than wildtype cells.

1. Kong, L.-J., Meloni, A. R., Nevins, J. R.The Rb-related p130 protein controls telomere lengthening through an interaction with a Rad50-interacting protein, RINT-1.Molec. Cell 22: 63-71, 2006. 2. Williams, J. P., Stewart, T., Li, B., Mulloy, R., Dimova, D., Classon, M.The retinoblastoma protein is required for Ras-induced oncogenic transformation.Molec. Cell. Biol. 26: 1170-1182, 2006. 3. Yeung, R. S., Bell, D. W., Testa, J. R., Mayol, X., Baldi, A., Grana, X., Klinga-Levan, K., Knudson, A. G., Giordano, A.The retinoblastoma-related gene, RB2, maps to human chromosome 16q12 and rat chromosome 19.Oncogene 8: 3465-3468, 1993.

RBL2

Retinoblastoma-like protein 2

retinoblastoma-like 2(p130)

130 kDa retinoblastoma associated protein antibody FLJ26459 antibody p130 antibody PRB 2 antibody PRB2 antibody Rb 2 antibody Rb2 antibody RBL 2 antibody RBL2 antibody RBR 2 antibody RBR2 antibody Retinoblastoma like 2 antibody Retinoblastoma like protein 2 antibody Retinoblastoma related gene antibody

Q08999