Boster Immunoleader

antibody

Anti-Leptin Receptor Antibody

PA1891

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse, rat

western blot, immunohistochemistry - paraffin section

Anti-Leptin Receptor Antibody

PA1891

Polyclonal

Rabbit IgG polyclonal antibody for Leptin receptor(LEPR) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, rat Predicted to work with: mouse

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Rat; Predicted Species: Human, Mouse

Concentration: 0.5-1μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

WB: The detection limit for LEPR is approximately 0.5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence in the middle region of human Leptin Receptor(798-817aa HDHFIPIEKYQFSLYPIFME), different from the related mouse and rat sequences by two amino acids.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

LEPR(Leptin receptor) also known as LEP-R, OBR, is a protein that in humans is encoded by the LEPR gene. The leptin hormone regulates adipose-tissue mass through hypothalamus effects on fullness and energy use. It acts through the leptin receptor(LEP-R), a single-transmembrane-domain receptor of the cytokine receptor family. By genetic mapping, Tartaglia et al.(1995) demonstrated that the Obr gene is located on mouse chromosome 4, in a 5.1-cM interval that contains the well-characterized recessive obesity mutation, diabetes(db). Chung et al.(1996) developed a genetic map of 1p in the region of the OBR gene. They mapped the OBR gene physically by radiation hybrid mapping and placed it on a contig composed of 10 adjacent YACs and 5 P1 artificial chromosomes(PACs). The location of the human homolog of Obr was predicted to be on 1p based on conserved linkage of most of the telomeric half of mouse chromosome 4 with human 1p. LEP-R functions as a receptor for the fat cell-specific hormone leptin. LEP-R has also been designated as CD295.

1. Chen, H., Charlat, O., Tartaglia, L. A., Woolf, E. A., Weng, X., Ellis, S. J., Lakey, N. D., Culpepper, J., Moore, K. J., Breitbart, R. E., Duyk, G. M., Tepper, R. I., Morgenstern, J. P.Evidence that the diabetes gene encodes the leptin receptor: identification of a mutation in the leptin receptor gene in db/db mice.Cell 84: 491-495, 1996. 2. Park, K. S., Shin, H. D., Park, B. L., Cheong, H. S., Cho, Y. M., Lee, H. K., Lee, J.-Y., Lee, J.-K., Oh, B., Kimm, K.Polymorphisms in the leptin receptor (LEPR)--putative association with obesity and T2DM.J. Hum. Genet. 51: 85-91, 2006. 3. Tartaglia, L. A., Dembski, M., Weng, X., Deng, N., Culpepper, J., Devos, R., Richards, G. J., Campfield, L. A., Clark, F. T., Deeds, J., Muir, C., Sanker, S., Moriarty, A., Moore, K. J., Smutko, J. S., Mays, G. G., Woolf, E. A., Monroe, C. A., Tepper, R. I.Identification and expression cloning of a leptin receptor, OB-R.Cell 83: 1263-1271, 1995.

LEPR

Leptin receptor

leptin receptor

CD 295 antibody CD295 antibody CD295 antigen antibody Db antibody Fa antibody HuB219 antibody LEP R antibody LEP-R antibody LEPR antibody LEPR_HUMAN antibody LEPRD antibody LEPROT antibody Leptin receptor antibody Leptin receptor fatty antibody Leptin receptor gene related protein antibody Leptin receptor precursor antibody OB R gene related protein antibody OB receptor antibody OB-R antibody OB-RGRP antibody obl antibody Obr antibody

P48357