Boster Immunoleader

antibody

Anti-KLF3 Antibody

PA1888

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse, rat

Anti-KLF3 Antibody

PA1888

Polyclonal

Rabbit IgG polyclonal antibody for Krueppel-like factor 3(KLF3) detection. Tested with WB in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, rat Predicted to work with: mouse

WB

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

WB: The detection limit for KLF3 is approximately 2.5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the N-terminus of human KLF3(20-34aa SYPSNYMESMKPNKY), different from the related rat sequence by one amino acid, and from the related mouse sequence by two amino acids.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

KLF3(Krueppel-like factor 3) is a protein that in humans is encoded by the KLF3 gene. It is also known as BKLF(Basic kruppel-like factor). KLF3 is a member of the Krüppel-like factors family of transcription factors. KLF3 primarily represses transcription through the transcriptional corepressor C-terminal binding protein(CtBP). Suske et al.(2005) stated that the human KLF3 gene maps to chromosome 4p14, and the mouse Klf3 gene maps to chromosome 5C3.3. Suske et al.(2005) stated that the human and mouse KLF3 proteins contain 345 and 344 amino acids, respectively. Both have a characteristic zinc finger domain, but lack the N-terminal buttonhead box found in related SP transcription factors. The repression mediated through KLF3 is also partially dependent on the sumoylation of its K10 and K197. Within KLF3's C-terminal, which is highly conserved throughout the KLF family, resides three C2H2 zinc fingers that preferentially bind the site NRG GNG NGR.

1. Suske, G., Bruford, E., Philipsen, S.Mammalian SP/KLF transcription factors: bring in the family.Genomics 85: 551-556, 2005. 2. Turner, Jeremy; Nicholas Hannah, Bishop David, Matthews Jacqueline M, Crossley Merlin "The LIM protein FHL3 binds basic Krüppel-like factor/Krüppel-like factor 3 and its co-repressor C-terminal-binding protein 2". J. Biol. Chem. (United States) 278 (15): 12786–95. 2003 3. Wang MJ, Qu XH, Wang LS, et al. "[cDNA cloning, subcellular localization and tissue expression of a new human Krüppel-like transcription factor: human basic Krüppel-like factor (hBKLF)]". Yi Chuan Xue Bao 30 (1): 1–9. 2003.

KLF3

Krueppel-like factor 3

Kruppel-like factor 3(basic)

Basic krueppel-like factor antibody Basic Kruppel like factor antibody BKLF3 antibody CACCC box binding protein BKLF antibody CACCC-box-binding protein BKLF antibody Klf3 antibody KLF3_HUMAN antibody Krueppel-like factor 3 antibody Kruppel like factor 3(basic) antibody MGC48279 antibody TEF-2 antibody TEF2 antibody Transcript ch138 antibody Zinc finger protein 741 antibody ZNF741 antibody

P57682