Anti-ErbB 3 Antibody | Boster Immunoleader PA1880 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-ErbB 3 Antibody

catalog :

PA1880

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human

application :

western blot

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-ErbB 3 Antibody

Catalog Number :

PA1880

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Receptor tyrosine-protein kinase erbB-3(ERBB3) detection. Tested with WB in Human.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human

Applications :

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Notes :

WB: The detection limit for ERBB3 is approximately 1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the N-terminus of human ErbB 3(162-181aa DWRDIVRDRDAEIVVKDNGR).

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Background :

ERBB3(V-erb-b2 avian erythroblastic leukemia viral oncogene homolog 3) also known as ONCOGENE ERBB3 or HER3, is an enzyme that in humans is encoded by the ERBB3 gene. This gene encodes a member of the epidermal growth factor receptor(EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. By in situ hybridization, Kraus et al.(1989) mapped the ERBB3 gene to chromosome 12q13. Carraway et al.(1994) demonstrated that ERBB3 is a receptor for heregulin and is capable of mediating HGL-stimulated tyrosine phosphorylation of itself. Most notably, EGFR and ErbB2 became markedly more promiscuous as the threshold was lowered, whereas ErbB3did not. Engelman et al.(2007) found that amplification of MET caused gefitinib resistance by driving ERBB3-dependent activation of phosphoinositide 3-kinase, a pathway though to be specific to EGFR/ERBB family receptors.

Reference :

1. Carraway, K. L., III, Sliwkowski, M. X., Akita, R., Platko, J. V., Guy, P. M., Nuijens, A., Diamonti, A. J., Vandlen, R. L., Cantley, L. C., Cerione, R. A. The erbB3gene product is a receptor for heregulin. J. Biol. Chem. 269: 14303-14306, 1994. 2. Engelman, J. A., Zejnullahu, K., Mitsudomi, T., Song, Y., Hyland, C., Park, J. O., Lindeman, N., Gale, C.-M., Zhao, X., Christensen, J., Kosaka, T., Holmes, A. J., Rogers, A. M., Cappuzzo, F., Mok, T., Lee, C., Johnson, B. E., Cantley, L. C, Janne, P. A. MET amplification leads to gefitinib resistance in lung cancer by activating ERBB3 signaling. Science 316: 1039-1043, 2007. 3. Kraus, M. H., Issing, W., Miki, T., Popescu, N. C., Aaronson, S. A. Isolation and characterization ofERBB3, a third member of the ERBB/epidermal growth factor receptor family: evidence for overexpression in a subset of human mammary tumors. Proc. Nat. Acad. Sci. 86: 9193-9197, 1989.

Gene Name :

ERBB3

Protein Name :

Receptor tyrosine-protein kinase erbB-3

Gene Full Name :

v-erb-b2 erythroblastic leukemia viral oncogene homolog 3(avian)

Synonyms :

c erbB 3 antibody c erbB3 antibody ERBB3 antibody ERBB3 protein antibody erbB3 S antibody ERBB3_HUMAN antibody Glial growth factor receptor antibody HER 3 antibody HER3 antibody LCCS2 antibody MDA BF 1 antibody MGC88033 antibody p180 ErbB3 antibody p45 sErbB3 antibody p85 sErbB3 antibody proto-oncogene-like protein c ErbB 3 antibody proto-oncogene-like protein c ErbB3 antibody Proto-oncogene-like protein c-ErbB-3 antibody receptor tyrosine protein kinase ERB3[precursor] antibody Receptor tyrosine protein kinase erbB 3 antibody Receptor tyrosine protein kinase erbB3 antibody Receptor tyrosine-protein kinase erbB-3 antibody Tyrosine kinase type cell surface receptor HER3 antibody Tyrosine kinase-type cell surface receptor HER3 antibody V erb b2 avian erythroblastic leukemia viral oncogene homolog 3 antibody v erb b2 erythroblastic leukemia viral oncogene homolog 3(avian) antibody v erb b2 erythroblastic leukemia viral oncogene homolog 3 antibody

Uniprot ID :

P21860

more info or order :

Boster Immunoleader product webpage