Boster Immunoleader

antibody

Anti-p57 Kip2 Antibody

PA1876

100 ug/vial

200 USD

polyclonal

rabbit

mouse, rat

Anti-p57 Kip2 Antibody

PA1876

Polyclonal

Rabbit IgG polyclonal antibody for Cyclin-dependent kinase inhibitor 1C(CDKN1C) detection. Tested with WB in Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: mouse, rat

WB

Concentration: 0.1-0.5μg/ml; Tested Species: Mouse, Rat

WB: The detection limit for CDKN1C is approximately 2.5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the N-terminus of mouse p57 Kip2(51-70aa RLAELNAEDQNRWDFNFQQD), identical to the related rat sequence, and different from the related human sequence by two amino acids.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

CDKN1C(Cyclin-dependent kinase inhibitor 1C) also called p57(KIP2) or KIP2, is a protein which in humans is encoded by the CDKN1C imprinted gene. The CDKN1C gene encodes p57(KIP2), a potent tight-binding inhibitor of several G1 cyclin/Cdk complexes and a negative regulator of cell proliferation. The CDKN1C gene is paternally imprinted, with preferential expression of the maternal allele. Matsuoka et al.(1995) demonstrated that the CDKN1C gene is located on chromosome 11p15.5, a region implicated in both sporadic cancers and Beckwith-Wiedemann syndrome, a familial cancer syndrome, making it a tumor suppressor candidate. The CDKN1C gene contains 3 exons. Mutations of CDKN1C are implicated in sporadic cancers and Beckwith-Wiedemann syndromesuggesting that it is a tumor suppressor candidate. Diaz-Meyer et al.(2005) concluded that CDKN1C may be downregulated by multiple mechanisms including some that do not involve promoter methylation.

1. Diaz-Meyer, N., Yang, Y., Sait, S. N., Maher, E. R., Higgins, M. J. Alternative mechanisms associated with silencing of CDKN1C in Beckwith-Wiedemann syndrome. J. Med. Genet. 42: 648-655, 2005. 2. Matsuoka, S., Edwards, M. C., Bai, C., Parker, S., Zhang, P., Baldini, A., Harper, J. W., Elledge, S. J.p57(KIP2), a structurally distinct member of the p21(CIP1) Cdk inhibitor family, is a candidate tumor suppressor gene. Genes Dev. 9: 650-662, 1995. 3. O'Keefe, D., Dao, D., Zhao, L., Sanderson, R., Warburton, D., Weiss, L., Anyane-Yeboa, K., Tycko, B. Coding mutations in p57(KIP2) are present in some cases of Beckwith-Wiedemann syndrome but are rare or absent in Wilms tumors. Am. J. Hum. Genet. 61: 295-303, 1997.

CDKN1C

Cyclin-dependent kinase inhibitor 1C

cyclin-dependent kinase inhibitor 1C(p57, Kip2)

Beckwith Wiedemann syndrome antibody BWCR antibody BWS antibody CDKI antibody CDKN 1C antibody CDKN1C antibody CDN1C_HUMAN antibody Cyclin dependent kinase inhibitor 1C antibody Cyclin dependent kinase inhibitor p57 antibody Cyclin-dependent kinase inhibitor 1C antibody Cyclin-dependent kinase inhibitor p57 antibody KIP 2 antibody KIP2 antibody p57 antibody p57 Kip 2 antibody p57Kip2 antibody WBS antibody

P49919