Anti-VCAM1 Antibody | Boster Immunoleader PA1873 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-VCAM1 Antibody

catalog :

PA1873

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

publications citing this reagent :

Chen Z, Zhang Y, Chen X, Liu X, Wang Z, Zhou X, et al. Retinoic Acid promotes interleukin-4 plasmid-dimethylsulfoxide topical transdermal delivery for treatment of psoriasis. Ann Dermatol. 2015;27:121-7 pubmed publisher
Teng X, Hu Z, Wei X, Wang Z, Guan T, Liu N, et al. IL-37 ameliorates the inflammatory process in psoriasis by suppressing proinflammatory cytokine production. J Immunol. 2014;192:1815-23 pubmed publisher
Yu M, Wang Z, Fang Y, Xiao M, Yuan Z, Lu C, et al. Overexpression of vasostatin-1 protects hypoxia/reoxygenation injuries in cardiomyocytes independent of endothelial cells. Cardiovasc Ther. 2012;30:145-51 pubmed publisher

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product information

Product Name :

Anti-VCAM1 Antibody

Catalog Number :

PA1873

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Vascular cell adhesion protein 1(VCAM1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, mouse, rat

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Mouse, Rat

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human, Mouse, Rat

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of mouse VCAM1(653-671aa YTIRQAQLQDAGIYECESK), different from the related human sequence by three amino acids, and from the related rat sequence by two amino acids.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

CD106(cluster of differentiation 106) also known as vascular cell adhesion molecule 1(VCAM-1),is a protein that in humans is encoded by the VCAM1 gene. In inflammatory conditions and in cardiac allografts undergoing rejection, VCAM1 is upregulated in endothelium of postcapillary venules. Arterial expression of VCAM1 is also found in experimental models of atherosclerosis in the rabbit. Cybulsky et al.(1991) mapped the VCAM1 gene to chromosome 1 by Southern analysis of somatic cell hybrids. Kumar et al.(1994) mapped the murine Vcam1 gene to chromosome 3 near Amy1.VCAM-1 functions as a cell adhesion molecule. The VCAM-1 protein mediates the adhesion of lymphocytes,monocytes, eosinophils, andbasophils to vascularendothelium. It also functions in leukocyte-endothelial cell signal transduction, and it may play a role in the development ofatherosclerosis and rheumatoid arthritis. CAM741 works similar to cotransin in that it represses the biosynthesis of VCAM1 cells by blocking the process of cotranslational translocation, which is dependent on the signal peptide ofVCAM1. Among the lung metastasis signature genes identified, several, includingVCAM1, were functionally validated.

Reference :

1. Cybulsky, M., Fries, J. W., Williams, A. J., Sultan, P., Eddy, R. L., Byers, M. G., Shows, T. B., Gimbrone, M. A., Jr., Collins, T. The human VCAM1 gene is assigned to chromosome 1p31-p32. (Abstract)Cytogenet. Cell Genet. 58: 1852, 1991. 2. Kumar, A. G., Dai, X. Y., Kozak, C. A., Mims, M. P., Gotto, A. M., Ballantyne, C. M. Murine VCAM-1: molecular cloning, mapping, and analysis of a truncated form. J. Immun. 153: 4088-4098, 1994. 3. Taylor, J. G., VI, Tang, D. C., Savage, S. A., Leitman, S. F., Heller, S. I., Serjeant, G. R., Rodgers, G. P., Chanock, S. J. Variants in the VCAM1 gene and risk for symptomatic stroke in sickle cell disease. Blood 100: 4303-4309, 2002.

Gene Name :

VCAM1

Protein Name :

Vascular cell adhesion protein 1

Gene Full Name :

vascular cell adhesion molecule 1

Synonyms :

CD 106 antibody CD106 antibody CD106 Antigen antibody DKFZp779G2333 antibody INCAM 100 antibody INCAM-100 antibody L1CAM antibody MGC99561 antibody V-CAM 1 antibody Vascular Cell Adhesion Molecule 1 antibody Vascular cell adhesion protein 1 antibody VCAM 1 antibody VCAM-1 antibody VCAM1 antibody VCAM1_HUMAN antibody

Uniprot ID :

P29533

more info or order :

Boster Immunoleader product webpage