Boster Immunoleader

antibody

Anti-beta Actin Antibody

PA1872

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse, rat

total: 13

1. Liu X, Qian L, Nan H, Cui M, Hao X, Du Y. Function of the transforming growth factor-β1/c-Jun N-terminal kinase signaling pathway in the action of thalidomide on a rat model of pulmonary fibrosis. Exp Ther Med. 2014;7:669-674 pubmed
2. Hou S, Ge K, Zheng X, Wei H, Sun R, Tian Z. CD226 protein is involved in immune synapse formation and triggers Natural Killer (NK) cell activation via its first extracellular domain. J Biol Chem. 2014;289:6969-77 pubmed publisher
3. Cui X, Liu J, Bai L, Tian J, Zhu J. Interleukin-6 induces malignant transformation of rat mesenchymal stem cells in association with enhanced signaling of signal transducer and activator of transcription 3. Cancer Sci. 2014;105:64-71 pubmed publisher
4. Su L, Ji J, Bian J, Fu Y, Ge Y, Yuan Z. Tacrolimus (FK506) prevents early retinal neovascularization in streptozotocin-induced diabetic mice. Int Immunopharmacol. 2012;14:606-12 pubmed publisher
5. Chu D, DU M, Hu X, Wu Q, Shen J. Paeoniflorin attenuates schistosomiasis japonica-associated liver fibrosis through inhibiting alternative activation of macrophages. Parasitology. 2011;138:1259-71 pubmed publisher
6. Meng Q, Chen X, Sun L, Zhao C, Sui G, Cai L. Carbamazepine promotes Her-2 protein degradation in breast cancer cells by modulating HDAC6 activity and acetylation of Hsp90. Mol Cell Biochem. 2011;348:165-71 pubmed publisher
7. Ni Y, Kuai J, Lu Z, Yang G, Fu H, Wang J, et al. Glycyrrhizin treatment is associated with attenuation of lipopolysaccharide-induced acute lung injury by inhibiting cyclooxygenase-2 and inducible nitric oxide synthase expression. J Surg Res. 2011;165:e29-35 pubmed publisher
8. Zhu M, Zhang J, Qu J, Zhang H, Zhou S, Dong S, et al. Up-regulation of growth factor independence 1 in endotoxin tolerant macrophages with low secretion of TNF-alpha and IL-6. Inflamm Res. 2010;59:855-60 pubmed publisher
9. Ni Y, Wang J, Yan X, Tian F, Zhao J, Wang Y, et al. Histone deacetylase inhibitor, butyrate, attenuates lipopolysaccharide-induced acute lung injury in mice. Respir Res. 2010;11:33 pubmed publisher
10. Shen L, Liu X, Hou W, Yang G, Wu Y, Zhang R, et al. NDRG2 is highly expressed in pancreatic beta cells and involved in protection against lipotoxicity. Cell Mol Life Sci. 2010;67:1371-81 pubmed publisher

Anti-beta Actin Antibody

PA1872

Polyclonal

Rabbit IgG polyclonal antibody for Actin, cytoplasmic 1(ACTB) detection. Tested with WB in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, rat Predicted to work with: mouse

WB

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the N-terminus of human beta Actin(2-19aaDDDIAALVVDNGSGMCKA), identical to the related rat and mouse sequences.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

beta-Actin(ACTB) also known as BETA-ACTIN or ACTIN, CYTOPLASMIC, 1, is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Ueyama et al.(1996) used fluorescence in situ hybridization to map ACTB to 7p22. By PCR of somatic cell hybrid DNAs, they mapped 4 ACTB pseudogenes to other chromosomes. Karakozova et al.(2006) found that arginylation of beta-actin regulates cell motility. The majority of Ate1-null cells appeared smaller than wildtype cells and were apparently unable to form a lamella during movement along the substrate. Karakozova et al.(2006) concluded that arginylation of beta-actin apparently represents a critical step in the actin N-terminal processing needed for actin functioning in vivo.

1. Gunning, P., Ponte, P., Okayama, H., Engel, J., Blau, H., Kedes, L. Isolation and characterization of full-length cDNA clones for human alpha-, beta-, and gamma-actin mRNAs: skeletal but not cytoplasmic actins have an amino-terminal cysteine that is subsequently removed. Molec. Cell. Biol. 3: 787-795, 1983. 2. Karakozova, M., Kozak, M., Wong, C. C. L., Bailey, A. O., Yates, J. R, III, Mogilner, A., Zebroski, H., Kashina, A. Arginylation of beta-actin regulates actin cytoskeleton and cell motility. Science 313: 192-196, 2006. 3. Ueyama, H., Inazawa, J., Nishino, H., Ohkubo, I., Miwa, T. FISH localization of human cytoplasmic actin genes ACTB to 7p22 and ACTG1 to 17q25 and characterization of related pseudogenes.Cytogenet. Cell Genet. 74: 221-224, 1996.

ACTB

Actin, cytoplasmic 1

actin, beta

A26C1A antibody A26C1B antibody ACTB antibody ACTB_HUMAN antibody Actin cytoplasmic 1 antibody Actx antibody b-actin antibody beta cytoskeletal actin antibody Beta-actin antibody E430023M04Rik antibody MGC128179 antibody PS1TP5 binding protein 1 antibody PS1TP5BP1 antibody

P60709