Anti-MRP4 Antibody | Boster Immunoleader PA1867 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-MRP4 Antibody

catalog :

PA1867

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot

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product information

Product Name :

Anti-MRP4 Antibody

Catalog Number :

PA1867

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Multidrug resistance-associated protein 4(ABCC4) detection. Tested with WB in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human Predicted to work with: mouse, rat

Applications :

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human; Predicted Species: Mouse, Rat

Notes :

WB: The detection limit for ABCC4 is approximately 1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the N-terminus of human MRP4(26-43aa WLNPLFKIGHKRRLEEDD), different from the related rat and mouse sequences by one amino acid.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Background :

ABCC4(Atp-binding cassette, subfamily c, member 4) also known as MRP4 or MOATB, is a protein that in humans is encoded by the ABCC4 gene. The ABCC4 belongs to a large family of transmembrane proteins involved in active transport of substrates out of cells. By radiation hybrid analysis, Kool et al.(1997) mapped the ABCC4 gene to chromosome 13. Using FISH, Lee et al.(1998) refined the localization to chromosome 13q32. ABCC4 acts as an independent regulator of intracellular cyclic nucleotide levels and as a mediator of cAMP-dependent signal transduction to the nucleus. The antiproliferative effect of MRP4 inhibition was related to cAMP-dependent PKA activation and CREB phosphorylation. Pharmacologic inhibition of MRP4 activity or knockdown of MRP4 via RNA interference resulted in reduced migration of DCs from human skin explants and of in vitro-generated Langerhans cells. Van de Ven et al.(2008) concluded that MRP4 contributes to migration of DCs toward draining lymph nodes and therefore has a role in the initiation of an immune response.

Reference :

1. Kool, M., de Haas, M., Scheffer, G. L., Scheper, R. J., van Eijk, M. J., Juijn, J. A., Baas, F., Borst, P. Analysis of expression of cMOAT (MRP2), MRP3, MRP4, and MRP5, homologues of the multidrug resistance-associated protein gene (MRP1), in human cancer cell lines. Cancer Res. 57: 3537-3547, 1997. 2. Lee, K., Belinsky, M. G., Bell, D. W., Testa, J. R., Kruh, G. D. Isolation of MOAT-B, a widely expressed multidrug resistance-associated protein/canalicular multispecific organic anion transporter-related transporter. Cancer Res. 58: 2741-2747, 1998. 3. van de Ven, R., Scheffer, G. L., Reurs, A. W., Lindenberg, J. J., Oerlemans, R., Jansen, G., Gillet, J.-P., Glasgow, J. N., Pereboev, A., Curiel, D. T., Scheper, R. J., de Gruijl, T. D. A role for multidrug resistance protein 4 (MRP4; ABCC4) in human dendritic cell migration.Blood 112: 2353-2359, 2008.

Gene Name :

ABCC4

Protein Name :

Multidrug resistance-associated protein 4

Gene Full Name :

ATP-binding cassette, sub-family C(CFTR/MRP), member 4

Synonyms :

ABCC 4 antibody ABCC4 antibody ATP binding cassette sub family C(CFTR/MRP) member 4 antibody ATP binding cassette sub family C member 4 antibody ATP binding cassette subfamily C member 4 antibody ATP-binding cassette sub-family C member 4 antibody bA464I2.1(ATP binding cassette, sub-family C(CFTR/MRP) member 4) antibody bA464I2.1 antibody Canalicular multispecific organic anion transporter ABC superfamily antibody canalicular multispecific organic anion transporter antibody EST170205 antibody MOAT B antibody MOAT-B antibody MOATB antibody MRP 4 antibody MRP cMOAT related ABC transporter antibody MRP/cMOAT related ABC transporter antibody MRP/cMOAT-related ABC transporter antibody MRP4_HUMAN antibody Multi specific organic anion transporter B antibody Multi-specific organic anion transporter B antibody Multidrug resistance associated protein 4 antibody Multidrug resistance-associated protein 4 antibody Multispecific organic anion tranporter B antibody OTTHUMP00000018560 antibody

Uniprot ID :

O15439

more info or order :

Boster Immunoleader product webpage